LAP (TGF-beta 1) Antibodies: Novus Biologicals

AF-246-NA

LAP (TGF-beta 1) Antibody [Unconjugated]

(3 Reviews)

100 μg

AF-246-NA

HAF017). A specific band was detected for LAP (TGF-beta 1) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for LAP (TGF-beta 1) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAP (TGF-beta 1) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

LAP (TGF-beta 1) was detected in immersion fixed Human peripheral blood mononuclear cells using Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LAP (TGF-beta 1) at approximately 61 kDa (as indicated) using 20 µg/mL of Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

246-LP) inhibits Recom-binant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recom-binant Human TGF-beta 1 (1 ng/mL) activity elicited by Recombin-ant Human LAP TGF-beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LAP TGF-beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA). The ND₅₀ is typically 0.4-2 µg/mL." class="big_lightbox">

Recombinant Human LAP TGF-beta 1 (Catalog # <a class=

246-LP) inhibits Recom-binant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recom-binant Human TGF-beta 1 (1 ng/mL) activity elicited by Recombin-ant Human LAP TGF-beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LAP TGF-beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA). The ND₅₀ is typically 0.4-2 µg/mL." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-246-NAU-100UG
Alexa Fluor 405	AF-246-NAV-100UG
Alexa Fluor 488	AF-246-NAG-100UG
Alexa Fluor 532	AF-246-NAX-100UG
Alexa Fluor 594	AF-246-NAT-100UG
Alexa Fluor 647	AF-246-NAR-100UG
Alexa Fluor 700	AF-246-NAN-100UG
Alexa Fluor 750	AF-246-NAS-100UG
Alexa Fluor Plus 405	AF-246-NAAFP405
Alexa Fluor Plus 488	AF-246-NAAFP488
Alexa Fluor Plus 555	AF-246-NAAFP555
Alexa Fluor Plus 594	AF-246-NAAFP594
Alexa Fluor Plus 647	AF-246-NAAFP647
Alexa Fluor Plus 680	AF-246-NAAFP680
Allophycocyanin	AF-246-NAA
Biotin	BAF246
CoraFluor 1	AF-246-NACL1
DyLight 350	AF-246-NAD
DyLight 405	AF-246-NAE
DyLight 488	AF-246-NAK
DyLight 550	AF-246-NAL
DyLight 594	AF-246-NAM
DyLight 650	AF-246-NAW
DyLight 680	AF-246-NAY
DyLight 755	AF-246-NAZ
FITC	AF-246-NAF
HRP	AF-246-NAH
Janelia Fluor 525	AF-246-NAJF525
Janelia Fluor 549	AF-246-NAI
Janelia Fluor 646	AF-246-NAJ
Janelia Fluor 669	AF-246-NAJF669
PE	AF-246-NAP
PerCP	AF-246-NAC
mFluor Violet 450 SE	AF-246-NAMFV450
mFluor Violet 500 SE	AF-246-NAMFV500
mFluor Violet 610 SE	AF-246-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-246-SP

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NBP2-22114

LAP (TGF-beta 1) Antibody (7F6) - BSA Free

(3 Reviews)

(21 Publications)

0.1 ml

NBP2-22114

Flow Cytometry: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Flow cytometric analysis of A549 cells using LAP (TGF-beta 1) mouse mAb (green) and negative control (red).

Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis of human stomach tissue (A) and human small intestine tissue (B) using TGF beta 1 antibody (NBP2-22114) at 2 ug/ml.

Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Human tonsil. Image from verified customer review.

Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.

ELISA: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

CD47 regulates thrombospondin-1, TGF-beta 1, and collagen deposition after injury. a Whole cell lysates from freshly isolated intestinal epithelial cells from Cd47f/f and Cd47 delta IEC mice were subjected to SDS–PAGE and immunoblot for thrombospondin-1/TSP-1, TGF-beta 1, and phosphorylated SMAD2 and SMAD3. Results are representative of three independent experiments. b Representative Masson’s trichrome staining of wounds beds and chronic DSS-colitis colons from Cd47f/f and Cd47 delta IEC mice. Scale bars = 50 μm. Results representative of three independent experiments with 5–7 mice per group. Source data are provided as a Source Data file Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31676794), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Inhibition of ERK1/2 activation attenuates myocardial remodeling and inflammation in permanent MI.Trametinib- and vehicle-treated mice were studied for 7 days after permanent LAD ligation. (A) Relative mRNA expression analysis of Il6, Tnf, Ccl2, and Ccr2 from the infarcted myocardium. (B) Flow cytometry analysis of the infarcted myocardium obtained from vehicle- or trametinib-treated mice normalized to heart weight. Representative gating strategies for quantification of CD45+ leukocytes: CD45+CD90.2–B220–NK1.1–CD11b+ myelomonocytic cells, CD45+CD90.2–B220–NK1.1–CD11b+Ly6G–F4/80–Ly6Chi monocytes, and CD45+CD90.2–B220–NK1.1–CD11b+Ly6G–F4/80–Ly6Clo macrophages. (C) Protein expression analysis of p-ERK1/2 (normalized to total ERK1/2) and activated TGF-beta 1 (normalized to GAPDH) in infarcted myocardium obtained from vehicle- or trametinib-treated mice. IOD, integrated optical density. (D) Protein expression analysis of p-ERK1/2 (normalized to ERK1/2) and activated TGF-beta 1 (normalized to GAPDH) in PBMCs isolated from vehicle- or trametinib-treated mice. Ordinary 1-way ANOVA, Šidak’s multiple-comparison test; n = 4–6 animals per group. Data are shown as mean +/- SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36548062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The upregulation of TGF beta 1/IGF-1/BDNF expression in the brain of AD mice by PBMT extended to promote adult hippocampal neurogenesis. A, B Western blotting analysis (A) and quantification (B) of TGF beta 1/IGF-1/BDNF expression in APP/PS1 and 3xTg-AD mouse brain after PBMT, (n = 3–4 per group). C The concentration of TGF beta 1/IGF-1/BDNF in brain tissues were measured by enzyme linked immunosorbent assay (ELISA), (n = 3–6 per group). D Representative images of Nestin+ (neural stem cell staining) and neuronal class-III beta -tubulin (Tuj1)+ (newborn neurons staining) expression cells in APP/PS1 and 3xTg-AD mouse hippocampal dentate gyrus (DG) at the end of PBMT. Scale bars, 50 μm. E Quantitative analyses of Nestin+ and Tuj1+ area in the hippocampal DG of each group, (n = 4 per group). F Quantitative analyses of the Nestin and Tuj1 mean fluorescence (MFI) in the brain tissues of each group after PBMT. The Nestin and Tuj1 MFI were detected by flow cytometer, (n = 5–6 per group). G Tuj1 antibody was used to staining the newborn neurons, and then, the expression of alpha -amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptors (AMPAR) and postsynaptic density protein 95 (PSD95) on Tuj1+ neurons were detected by flow cytometer. All quantifications are presented as mean +/- SEM and were analyzed by one-way ANOVA test; ***p < 0.001, **p < 0.01, *p < 0.05 versus WT group; ###p < 0.001, ##p < 0.01, #p < 0.05 versus indicated group Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36217178), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

TF cytoplasmic domain phosphorylation–dependent increased TGF-beta 1 activation in clinical setting of MI.(A) Representative confocal images of phosphorylation status of TF in infarcted myocardium obtained from WT or TF delta CT mice after 7 days. Representative images and quantification of biological replicates. Kruskal-Wallis test and Dunn’s multiple-comparison test; n = 3–4 animals per group. Scale bars: 50 μm. (B) Representative immunofluorescence confocal microscopy images of CD45+ and CD45/p-TF double-positive cells in human myocardium specimens obtained from n = 5 nonischemic (NI) donor hearts and n = 7 IHF patients. Quantification of biological replicates. Mann-Whitney test. Scale bars: 50 μm. (C and D) Western blot analysis and quantification of human LV tissue obtained from n = 5 nonischemic donor hearts and n = 9 IHF patients for p-TF (normalized to total TF) and TF (C) or TGF-beta 1 (normalized to GAPDH) and p-SMAD2 (normalized to total SMAD2) (D). Mann-Whitney test. Data are shown as mean +/- SEM. *P < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36548062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

A profibrotic MEK1/2–TGF-beta 1 pathway is linked to PAR2-mediated ROS signaling in monocytes.(A and B) Protein expression analysis of monocytes isolated from WT mice and pretreated in vitro with trametinib (10 μM) for 1 hour (A), or isolated from PAR2–/– versus WT mice (B). Cells were stimulated with an inflammatory cytokine cocktail containing IL-6, TNF-alpha , and CCL2 at a concentration of 20 ng/mL with and without hypoxia for 4 hours. Western blotting of p-ERK1/2 (normalized to total ERK1/2) and activated TGF-beta 1 (normalized to GAPDH). Ordinary 1-way ANOVA, Šidak’s multiple-comparison test; n = 5 replicates (2–3 mice were pooled for each sample). (C–E) PAR2fl/fl and PAR2fl/fl LysMCre littermates were subjected to permanent LAD ligation and investigated after 7 days; n = 5–10 animals per group. (C) Western blot analysis of activated TGF-beta 1 (normalized to GAPDH) and p-SMAD2 (normalized to total SMAD2) in the infarcted myocardium. Representative blots and quantification of biological replicates. (D) High-frequency ultrasound echocardiography obtained from PAR2fl/fl LysMCre and PAR2fl/fl littermate control mice with measurement of LVEF (%) and LVEDV (μL). Mann-Whitney test. (E) Kaplan-Meier survival analysis of permanently LAD-ligated PAR2fl/fl LysMCre and PAR2fl/fl littermate control mice over 7 days. Log-rank (Mantel-Cox) test. (F) Sirius red staining and deconvoluted images of fibrotic area on paraffin-embedded heart sections 4 weeks after permanent LAD ligation to induce IHF. Representative images and quantification of fibrotic areas normalized to surface area. Unpaired, 2-sided t test; n = 5 animals per group. Data are shown as mean +/- SEM. *P < 0.05, **P < 0.01, ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36548062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Myeloid cell–derived TF-PAR2 complex is required for TGF-beta 1 activation.(A) Confocal microscopy of myocardial cryosections obtained from n = 5 sham-operated and n = 5 LAD-ligated WT (C57BL/6J) mice at day 7. Representative images and quantification of TF+ cells costained for CD45. Unpaired, 2-sided t test. Scale bar: 50 μm. (B–D) TFfl/fl LysMCre and TFfl/fl littermates were subjected to permanent LAD ligation versus sham surgery and investigated after 7 days; n = 5–7 animals per group. (B) Protein expression analysis of p-ERK1/2 (normalized to total ERK1/2) in the infarcted myocardium. Ordinary 1-way ANOVA, Šidak’s multiple-comparison test. (C) Western blot analysis of activated TGF-beta 1 (normalized to GAPDH) and p-SMAD2 (normalized to total SMAD2) in the infarcted myocardium obtained from TFfl/fl LysMCre and TFfl/fl littermates. Representative blots and quantification of biological replicates. (D) High-frequency ultrasound echocardiography obtained from TFfl/fl LysMCre and TFfl/fl littermates. Ordinary 1-way ANOVA, Šidak’s multiple-comparison test. (E) Sirius red staining and deconvoluted images of fibrotic area on paraffin-embedded heart sections 4 weeks after permanent LAD ligation to induce IHF versus sham surgery. Representative images and quantification of fibrotic areas normalized to surface area. Ordinary 1-way ANOVA, Šidak’s multiple-comparison test; n = 5 animals per group. (F) Kaplan-Meier survival analysis of permanently LAD-ligated TFfl/fl LysMCre and TFfl/fl littermate mice over 4 weeks. Log-rank (Mantel-Cox) test; n = 10–15 animals per group. Data are shown as mean +/- SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36548062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Myeloid cell TF cytoplasmic domain phosphorylation mediates ERK1/2–TGF-beta 1–dependent cardiac remodeling in permanent LAD ligation.(A) Confocal microscopy of myocardial cryosections obtained from WT (C57BL/6J) and TF delta CT mice. Representative images and quantification of MFI of Ly6C+TGF beta -1+ and CD31+TGF-beta 1+ cells. Ordinary 1-way ANOVA, Šidak’s multiple-comparison test; n = 5 animals per group. Scale bars: 25 μm. (B) Mice with transplanted BM were subjected to permanent LAD ligation versus sham surgery and investigated 7 days later. Western blot analysis of NOX2 (normalized to GAPDH), p-ERK1/2 (normalized to total ERK1/2), and TGF-beta 1 (normalized to GAPDH) in infarcted myocardium obtained from chimeric mice. Ordinary 1-way ANOVA, Šidak’s multiple-comparison test; n = 5–7 animals per group. Data are shown as mean +/- SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36548062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22114AF350
Alexa Fluor 405	NBP2-22114AF405
Alexa Fluor 488	NBP2-22114AF488
Alexa Fluor 532	NBP2-22114AF532
Alexa Fluor 594	NBP2-22114AF594
Alexa Fluor 647	NBP2-22114AF647
Alexa Fluor 700	NBP2-22114AF700
Alexa Fluor 750	NBP2-22114AF750
Alexa Fluor Plus 405	NBP2-22114AFP405
Alexa Fluor Plus 488	NBP2-22114AFP488
Alexa Fluor Plus 555	NBP2-22114AFP555
Alexa Fluor Plus 594	NBP2-22114AFP594
Alexa Fluor Plus 647	NBP2-22114AFP647
Alexa Fluor Plus 680	NBP2-22114AFP680
Biotin	NBP2-22114B
CoraFluor 1	NBP2-22114CL1
DyLight 350	NBP2-22114UV
DyLight 405	NBP2-22114V
DyLight 488	NBP2-22114G
DyLight 550	NBP2-22114R
DyLight 594	NBP2-22114DL594
DyLight 650	NBP2-22114C
DyLight 680	NBP2-22114FR
DyLight 755	NBP2-22114IR
FITC	NBP2-22114F
HRP	NBP2-22114H
Janelia Fluor 525	NBP2-22114JF525
Janelia Fluor 549	NBP2-22114JF549
Janelia Fluor 646	NBP2-22114JF646
Janelia Fluor 669	NBP2-22114JF669
mFluor Violet 450 SE	NBP2-22114MFV450
mFluor Violet 500 SE	NBP2-22114MFV500
mFluor Violet 610 SE	NBP2-22114MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22114SS
Azide and BSA Free	NBP2-80827

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AB-246-NA

LAP (TGF-beta 1) Antibody [Unconjugated]

(1 Review)

(11 Publications)

1 mg

AB-246-NA

NL001) and counter-stained with DAPI (blue).View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

TGF-beta 1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human LAP TGF-beta 1 Polyclonal Antibody (Catalog # AB-246-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # <a class=

NL001) and counter-stained with DAPI (blue).View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="TGF-beta 1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human LAP TGF-beta 1 Polyclonal Antibody (Catalog # AB-246-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue).View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

246-LP) inhibits Recombinant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF-beta 1 (0.25 ng/mL) activity elicited by Recombinant Human LAP TGF-beta 1 (125 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human LAP TGF-beta 1 Polyclonal Antibody (Catalog # AB-246-NA). The ND₅₀ is typically 15-30 µg/mL." class="big_lightbox">

246-LP) inhibits Recombinant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF-beta 1 (0.25 ng/mL) activity elicited by Recombinant Human LAP TGF-beta 1 (125 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human LAP TGF-beta 1 Polyclonal Antibody (Catalog # AB-246-NA). The ND₅₀ is typically 15-30 µg/mL." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

LAP (TGF-beta 1) was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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MAB246

LAP (TGF-beta 1) Antibody (27235) [Unconjugated]

(13 Publications)

100 μg

MAB246

NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

LAP (TGF-beta 1) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB246) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # <a class=

NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="LAP (TGF-beta 1) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB246) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

246-LP) inhibits Recombinant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF-beta 1 (1 ng/mL) activity elicited by Recombinant Human LAP TGF-beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human LAP TGF-beta 1 Monoclonal Antibody (Catalog # MAB246). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox">

246-LP) inhibits Recombinant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF-beta 1 (1 ng/mL) activity elicited by Recombinant Human LAP TGF-beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human LAP TGF-beta 1 Monoclonal Antibody (Catalog # MAB246). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

Specificity of R58 and L59 antibodies. (a) Schematic structure of a GST- rhLTGF-beta 1 protein generated in bacteria and a commercial rhLAP beta 1 generated in insect cells. The PLK cleavage site is indicated by arrowhead. (b-e) GST-LTGF-beta 1/rhLAP beta 1was incubated with/without PLK or PLN for 45 min at 37°C, and thereafter equal amounts of samples containing either 50 ng GST-rhLTGF-beta 1 or 500 ng rhLAP beta 1 were subjected to each lane in SDS-PAGE under reducing conditions followed by Western blot analyses using R58/L59 antibodies (b, d, respectively). Membranes used in panels b and d were reprobed with monoclonal (c) or polyclonal (e) anti-LAP antibodies. Lanes 1, intact GST-rhLTGF-beta 1 or rhLAP-beta 1; lanes 2, PLK digested GST-rhLTGF-beta 1 or rhLAP-beta 1; lanes 3, PLK; lane 4, PLN digested GST-rhLTGF-beta 1 or rhLAP-beta 1; lane 5, PLN. Representative results from three independent experiments with a similar result are shown. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24877031), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB246U-100UG
Alexa Fluor 405	FAB246V-100UG
Alexa Fluor 488	FAB246G-100UG
Alexa Fluor 532	FAB246X-100UG
Alexa Fluor 594	FAB246T-100UG
Alexa Fluor 647	FAB246R-100UG
Alexa Fluor 700	FAB246N-100UG
Alexa Fluor 750	FAB246S-100UG
Alexa Fluor Plus 405	FAB246AFP405-100UG
Alexa Fluor Plus 488	FAB246AFP488-100UG
Alexa Fluor Plus 555	FAB246AFP555-100UG
Alexa Fluor Plus 594	FAB246AFP594-100UG
Alexa Fluor Plus 647	FAB246AFP647-100UG
Alexa Fluor Plus 680	FAB246AFP680-100UG
Allophycocyanin	FAB246A
Biotin	FAB246B
CoraFluor 1	FAB246CL1
DyLight 350	FAB246D
DyLight 405	FAB246E
DyLight 488	FAB246K
DyLight 550	FAB246L
DyLight 594	FAB246M
DyLight 650	FAB246W
DyLight 680	FAB246Y
DyLight 755	FAB246Z
FITC	FAB246F
HRP	FAB246H
Janelia Fluor 525	FAB246JF525
Janelia Fluor 549	FAB246I
Janelia Fluor 646	FAB246J
Janelia Fluor 669	FAB246JF669
PE	FAB246P
PerCP	FAB246C
mFluor Violet 450 SE	FAB246MFV450
mFluor Violet 500 SE	FAB246MFV500
mFluor Violet 610 SE	FAB246MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB246-SP
Carrier Free	MAB246-500

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MAB2463

LAP (TGF-beta 1) Antibody (27232) [Unconjugated]

(10 Publications)

500 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2463U-100UG
Alexa Fluor 405	FAB2463V-100UG
Alexa Fluor 488	FAB2463G
Alexa Fluor 532	FAB2463X
Alexa Fluor 594	FAB2463T-100UG
Alexa Fluor 647	FAB2463R-100UG
Alexa Fluor 750	FAB2463S-100UG
Alexa Fluor Plus 405	FAB2463AFP405-100UG
Alexa Fluor Plus 488	FAB2463AFP488-100UG
Alexa Fluor Plus 555	FAB2463AFP555-100UG
Alexa Fluor Plus 594	FAB2463AFP594-100UG
Alexa Fluor Plus 647	FAB2463AFP647-100UG
Alexa Fluor Plus 680	FAB2463AFP680-100UG
Allophycocyanin	FAB2463A
Allophycocyanin/Cy7	FAB2463APCCY7
Biotin	FAB2463B
CoraFluor 1	FAB2463CL1
DyLight 350	FAB2463D
DyLight 405	FAB2463E
DyLight 488	FAB2463K
DyLight 550	FAB2463L
DyLight 594	FAB2463M
DyLight 650	FAB2463W
DyLight 680	FAB2463Y
DyLight 755	FAB2463Z
FITC	FAB2463F
HRP	FAB2463H
Janelia Fluor 525	FAB2463JF525
Janelia Fluor 549	FAB2463I
Janelia Fluor 646	FAB2463J
Janelia Fluor 669	FAB2463JF669
PE/Atto594	FAB2463PEATT594
PE/Cy5.5	FAB2463PECY55
PE/Cy7	FAB2463PECY7
Phycoerythrin	FAB2463P
mFluor Violet 450 SE	FAB2463MFV450
mFluor Violet 500 SE	FAB2463MFV500
mFluor Violet 610 SE	FAB2463MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB2463A
Carrier Free	FAB2463G
Carrier Free	FAB2463P
Carrier Free	MAB2463-SP
Carrier Free	FAB2463V-100UG
Carrier Free	FAB2463U-100UG
Carrier Free	FAB2463T-100UG
Carrier Free	FAB2463S-100UG
Carrier Free	FAB2463R-100UG

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MAB77541

LAP (TGF-beta 1) Antibody (1015022) [Unconjugated]

100 μg

MAB77541

MAB0041, open histogram) followed by APC-conjugated anti-Mouse IgG secondary antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

MAB0041, open histogram) followed by APC-conjugated anti-Mouse IgG secondary antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." alt="PC-3 human prostate cancer cell line was stained with Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by APC-conjugated anti-Mouse IgG secondary antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

LAP (TGF-beta 1) was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

DY990). Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB7754) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox">

Recombinant Human LAP (TGF-beta 1) protein was serially diluted 2-fold and captured by Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541) coated on a Clear Polystyrene Microplate (Catalog # <a class=

DY990). Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB7754) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC77541U-100UG
Alexa Fluor 405	IC77541V-100UG
Alexa Fluor 488	IC77541G-100UG
Alexa Fluor 532	FAB77541X
Alexa Fluor 594	IC77541T-100UG
Alexa Fluor 647	IC77541R-100UG
Alexa Fluor 700	IC77541N-100UG
Alexa Fluor 750	IC77541S-100UG
Alexa Fluor Plus 405	FAB77541AFP405-100UG
Alexa Fluor Plus 488	FAB77541AFP488-100UG
Alexa Fluor Plus 555	FAB77541AFP555-100UG
Alexa Fluor Plus 594	FAB77541AFP594-100UG
Alexa Fluor Plus 647	FAB77541AFP647-100UG
Alexa Fluor Plus 680	FAB77541AFP680-100UG
Allophycocyanin	FAB77541A
Biotin	FAB77541B
CoraFluor 1	FAB77541CL1
DyLight 350	FAB77541D
DyLight 405	FAB77541E
DyLight 488	FAB77541K
DyLight 550	FAB77541L
DyLight 594	FAB77541M
DyLight 650	FAB77541W
DyLight 680	FAB77541Y
DyLight 755	FAB77541Z
FITC	FAB77541F
HRP	FAB77541H
Janelia Fluor 525	FAB77541JF525
Janelia Fluor 549	FAB77541I
Janelia Fluor 646	FAB77541J
Janelia Fluor 669	FAB77541JF669
PE	FAB77541P
PerCP	FAB77541C
mFluor Violet 450 SE	FAB77541MFV450
mFluor Violet 500 SE	FAB77541MFV500
mFluor Violet 610 SE	FAB77541MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB77541-SP
Carrier Free	IC77541V-100UG
Carrier Free	IC77541U-100UG
Carrier Free	IC77541T-100UG
Carrier Free	IC77541S-100UG
Carrier Free	IC77541R-100UG
Carrier Free	IC77541N-100UG
Carrier Free	IC77541G-100UG

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MAB2461

LAP (TGF-beta 1) Antibody (9005)

(2 Publications)

100 μg

MAB2461

246-LP) was serially diluted and captured by Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB2461) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF-beta 1) Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox">

246-LP) was serially diluted and captured by Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB2461) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF-beta 1) Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Human LAP (TGF-beta 1) (Catalog # 246-LP) was serially diluted and captured by Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB2461) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF-beta 1) Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2461U-100UG
Alexa Fluor 405	FAB2461V-100UG
Alexa Fluor 488	FAB2461G-100UG
Alexa Fluor 532	FAB2461X-100UG
Alexa Fluor 594	FAB2461T-100UG
Alexa Fluor 647	FAB2461R-100UG
Alexa Fluor 700	FAB2461N-100UG
Alexa Fluor 750	FAB2461S-100UG
Alexa Fluor Plus 405	FAB2461AFP405-100UG
Alexa Fluor Plus 488	FAB2461AFP488-100UG
Alexa Fluor Plus 555	FAB2461AFP555-100UG
Alexa Fluor Plus 594	FAB2461AFP594-100UG
Alexa Fluor Plus 647	FAB2461AFP647-100UG
Alexa Fluor Plus 680	FAB2461AFP680-100UG
Allophycocyanin	FAB2461A
Biotin	FAB2461B
CoraFluor 1	FAB2461CL1
DyLight 350	FAB2461D
DyLight 405	FAB2461E
DyLight 488	FAB2461K
DyLight 550	FAB2461L
DyLight 594	FAB2461M
DyLight 650	FAB2461W
DyLight 680	FAB2461Y
DyLight 755	FAB2461Z
FITC	FAB2461F
HRP	FAB2461H
Janelia Fluor 525	FAB2461JF525
Janelia Fluor 549	FAB2461I
Janelia Fluor 646	FAB2461J
Janelia Fluor 669	FAB2461JF669
PE	FAB2461P
PerCP	FAB2461C
mFluor Violet 450 SE	FAB2461MFV450
mFluor Violet 500 SE	FAB2461MFV500
mFluor Violet 610 SE	FAB2461MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2461-SP
Carrier Free	MAB2461-500

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MAB7754

LAP (TGF-beta 1) Antibody (1015016) [Unconjugated]

100 μg

MAB7754

MAB77541) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB7754) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox">

MAB77541) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB7754) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human LAP (TGF-beta 1) protein was serially diluted 2-fold and captured by Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB7754) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7754U-100UG
Alexa Fluor 405	FAB7754V-100UG
Alexa Fluor 488	FAB7754G-100UG
Alexa Fluor 532	FAB7754X-100UG
Alexa Fluor 594	FAB7754T-100UG
Alexa Fluor 647	FAB7754R-100UG
Alexa Fluor 700	FAB7754N-100UG
Alexa Fluor 750	FAB7754S-100UG
Alexa Fluor Plus 405	FAB7754AFP405-100UG
Alexa Fluor Plus 488	FAB7754AFP488-100UG
Alexa Fluor Plus 555	FAB7754AFP555-100UG
Alexa Fluor Plus 594	FAB7754AFP594-100UG
Alexa Fluor Plus 647	FAB7754AFP647-100UG
Alexa Fluor Plus 680	FAB7754AFP680-100UG
Allophycocyanin	FAB7754A
Biotin	FAB7754B
CoraFluor 1	FAB7754CL1
DyLight 350	FAB7754D
DyLight 405	FAB7754E
DyLight 488	FAB7754K
DyLight 550	FAB7754L
DyLight 594	FAB7754M
DyLight 650	FAB7754W
DyLight 680	FAB7754Y
DyLight 755	FAB7754Z
FITC	FAB7754F
HRP	FAB7754H
Janelia Fluor 525	FAB7754JF525
Janelia Fluor 549	FAB7754I
Janelia Fluor 646	FAB7754J
Janelia Fluor 669	FAB7754JF669
PE	FAB7754P
PerCP	FAB7754C
mFluor Violet 450 SE	FAB7754MFV450
mFluor Violet 500 SE	FAB7754MFV500
mFluor Violet 610 SE	FAB7754MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7754-SP

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NBP3-21360

LAP (TGF-beta 1) Antibody - BSA Free

100 ul

NBP3-21360

Staining of human cell line U-2 OS shows localization to cytosol & the Golgi apparatus.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21360-25ul

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MAB7666

LAP (TGF-beta 1) Antibody (860206) [Unconjugated]

(12 Publications)

100 μg

MAB7666

240-B), and 20 µg/mL Recombinant Mouse IL-2 (Catalog # 402-IL) for 24 hours to induce T regulatory cells (iTregs) were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB7666) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_lightbox">

<b>Biological Strategies Validation. </b>Mouse splenocytes treated with 10 µg/mL Anti-CD3, 5 µg/mL Anti-CD28, 10 µg/mL Recombinant Human TGF-beta 1 (Catalog # <a class=

240-B), and 20 µg/mL Recombinant Mouse IL-2 (Catalog # 402-IL) for 24 hours to induce T regulatory cells (iTregs) were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB7666) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." alt="Mouse splenocytes treated with 10 µg/mL Anti-CD3, 5 µg/mL Anti-CD28, 10 µg/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 µg/mL Recombinant Mouse IL-2 (Catalog # 402-IL) for 24 hours to induce T regulatory cells (iTregs) were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB7666) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7666U-100UG
Alexa Fluor 405	FAB7666V-100UG
Alexa Fluor 488	FAB7666G-100UG
Alexa Fluor 532	FAB7666X
Alexa Fluor 594	FAB7666T-100UG
Alexa Fluor 647	FAB7666R-100UG
Alexa Fluor 700	FAB7666N-100UG
Alexa Fluor 750	FAB7666S-100UG
Alexa Fluor Plus 405	FAB7666AFP405-100UG
Alexa Fluor Plus 488	FAB7666AFP488-100UG
Alexa Fluor Plus 555	FAB7666AFP555-100UG
Alexa Fluor Plus 594	FAB7666AFP594-100UG
Alexa Fluor Plus 647	FAB7666AFP647-100UG
Alexa Fluor Plus 680	FAB7666AFP680-100UG
Allophycocyanin	FAB7666A
Allophycocyanin/Cy7	FAB7666APCCY7
Biotin	FAB7666B
CoraFluor 1	FAB7666CL1
DyLight 350	FAB7666D
DyLight 405	FAB7666E
DyLight 488	FAB7666K
DyLight 550	FAB7666L
DyLight 594	FAB7666M
DyLight 650	FAB7666W
DyLight 680	FAB7666Y
DyLight 755	FAB7666Z
FITC	FAB7666F
HRP	FAB7666H
Janelia Fluor 525	FAB7666JF525
Janelia Fluor 549	FAB7666I
Janelia Fluor 646	FAB7666J
Janelia Fluor 669	FAB7666JF669
PE	FAB7666P
PE/Atto594	FAB7666PEATT594
PE/Cy5.5	FAB7666PECY55
PE/Cy7	FAB7666PECY7
PerCP	FAB7666C
mFluor Violet 450 SE	FAB7666MFV450
mFluor Violet 500 SE	FAB7666MFV500
mFluor Violet 610 SE	FAB7666MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7666-SP
Carrier Free	FAB7666V-100UG
Carrier Free	FAB7666U-100UG
Carrier Free	FAB7666T-100UG
Carrier Free	FAB7666S-100UG
Carrier Free	FAB7666R-100UG
Carrier Free	FAB7666N-100UG
Carrier Free	FAB7666G-100UG

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BAM2462

LAP (TGF-beta 1) Antibody (27240) [Biotin]

(1 Review)

(5 Publications)

50 μg

BAM2462

246-LP) was serially diluted and captured by Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB2461) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF-beta 1) Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

246-LP) was serially diluted and captured by Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB2461) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF-beta 1) Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human LAP (TGF-beta 1) (Catalog # 246-LP) was serially diluted and captured by Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB2461) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF-beta 1) Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Biotin

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FAB7754AFP647

LAP (TGF-beta 1) Antibody (1015016) [Alexa Fluor™ Plus 647]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 647

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

LAP (TGF-beta 1) Antibodies

LAP (TGF-beta 1) Antibody [Unconjugated]

LAP (TGF-beta 1) Antibody (7F6) - BSA Free

LAP (TGF-beta 1) Antibody [Unconjugated]

LAP (TGF-beta 1) Antibody (27235) [Unconjugated]

LAP (TGF-beta 1) Antibody (27232) [Unconjugated]

LAP (TGF-beta 1) Antibody (1015022) [Unconjugated]

LAP (TGF-beta 1) Antibody (9005)

LAP (TGF-beta 1) Antibody (1015016) [Unconjugated]

LAP (TGF-beta 1) Antibody - BSA Free

LAP (TGF-beta 1) Antibody (860206) [Unconjugated]

LAP (TGF-beta 1) Antibody (27240) [Biotin]

LAP (TGF-beta 1) Antibody (1015016) [Alexa Fluor™ Plus 647]