TGF-alpha Antibody (MF9) [Janelia Fluor® 646]

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC, CyTOF-ready
• Clone: MF9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 646

TGF-alpha Antibody (MF9) [Janelia Fluor® 646] Summary

• Immunogen: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF-alpha (exact sequence is proprietary) (Uniprot: P01135)
• Localization: Cytoplasmic and Extracellular (secreted)
• Specificity: This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGFA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for TGF-alpha Antibody (MF9) [Janelia Fluor® 646]

• protransforming growth factor alpha
• TFGA
• TGFA
• TGFalpha
• TGF-alpha
• transforming growth factor, alpha
• transforming growth factor-alpha

Background

Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-alpha Antibody (NBP2-34683JF646)(1)

Publication using NBP2-34683JF646

• Lö beta lein L, Linnerbauer M, Zuber F et al. TGF alpha controls checkpoints in CNS resident and infiltrating immune cells to promote resolution of inflammation. Nature Communications 2025-06-19 [PMID: 40537468]

Bioinformatics

• Gene Symbol: TGFA