TFPI Antibody

Western Blot: TFPI Antibody [NB100-786] - Western blot analysis of TFPI in Hela lysate using NB100-786 at 0.05 ug/ ml.

Western Blot: TFPI Antibody [NB100-786] - HEK293 overexpressing TFPI and probed with (mock transfection in first lane).

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TFPI Antibody Summary

• Description: Novus Biologicals Goat TFPI Antibody (NB100-786) is a polyclonal antibody validated for use in WB and ELISA. Anti-TFPI Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-VKIAYEEIFVKNM corresponding to C-Terminus according to NP_006278.1.
• Specificity: This antibody is expected to recognize isoform a (NP_006278.1) only.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TFPI
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in lysates of HepG2 and HeLa (calculated MW of 35.0 kDa band according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approx. 38 kDa band to 45 kDa band are observed. These bands are not observed in the non-transfected HEK293.
• Control:

  HeLa Whole Cell Lysate
  TFPI Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TFPI Antibody

• convertin
• EPI
• EPITFPI1LACITFI
• Extrinsic pathway inhibitor
• LACI
• Lipoprotein-associated coagulation inhibitor
• TFPI
• tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• tissue factor pathway inhibitor

Background

A protease inhibitor that inhibits fibrin clot formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFPI Antibody (NB100-786)(1)

Publication using NB100-786

• Wun TC, Kretzmer KK, Girard TJ et al. Cloning and characterization of a cDNA coding for the lipoprotein-associated coagulation inhibitor shows that it consists of three tandem Kunitz-type inhibitory domains. J Biol Chem 1988 May 5;263(13):6001-4 1988-05-05 [PMID: 2452157]

Bioinformatics

• Gene Symbol: TFPI
• Entrez:
  • 7035(Human)
• Uniprot:
  • NP_006278.(Human)