HAF019). A specific band was detected for TFPI at approximately 40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2974) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TFPI at approximately 40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2974-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74493 | |||
HAF017). A specific band was detected for TFPI at approximately 37-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of mouse placenta tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2975) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TFPI at approximately 37-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2975-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84831-25ul | |||
2974-PI) was serially diluted and captured by Mouse Anti-Human TFPI Monoclonal Antibody (Catalog # MAB29741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2974) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human TFPI (Catalog # 2974-PI) was serially diluted and captured by Mouse Anti-Human TFPI Monoclonal Antibody (Catalog # MAB29741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2974) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB29741-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2975-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2974-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89570-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89569-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26290-50ul | |||
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
2974-PI) was serially diluted and captured by Mouse Anti-Human TFPI Monoclonal Antibody (Catalog # ![Western Blot: TFPI Antibody (5H5) [NBP2-46098] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFPI.](http://images.novusbio.com/fullsize/TFPI-Antibody-5H5-Western-Blot-NBP2-46098-img0009.jpg "Western Blot: TFPI Antibody (5H5) [NBP2-46098] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFPI.")
![Immunohistochemistry: TFPI Antibody (5H5) [NBP2-46098] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TFPI-Antibody-5H5-Immunohistochemistry-NBP2-46098-img0008.jpg)
![Immunohistochemistry: TFPI Antibody (5H5) [NBP2-46098] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TFPI-Antibody-5H5-Immunohistochemistry-NBP2-46098-img0001.jpg)
![Immunohistochemistry: TFPI Antibody (5H5) [NBP2-46098] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TFPI-Antibody-5H5-Immunohistochemistry-NBP2-46098-img0002.jpg)
![Immunohistochemistry: TFPI Antibody (5H5) [NBP2-46098] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TFPI-Antibody-5H5-Immunohistochemistry-NBP2-46098-img0003.jpg)
![Immunohistochemistry: TFPI Antibody (5H5) [NBP2-46098] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TFPI-Antibody-5H5-Immunohistochemistry-NBP2-46098-img0004.jpg)
![Immunohistochemistry: TFPI Antibody (5H5) [NBP2-46098] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TFPI-Antibody-5H5-Immunohistochemistry-NBP2-46098-img0005.jpg)
![Immunohistochemistry: TFPI Antibody (5H5) [NBP2-46098] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TFPI-Antibody-5H5-Immunohistochemistry-NBP2-46098-img0006.jpg)
![Immunohistochemistry: TFPI Antibody (5H5) [NBP2-46098] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TFPI-Antibody-5H5-Immunohistochemistry-NBP2-46098-img0007.jpg)
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![Immunohistochemistry-Paraffin: TFPI Antibody [NBP1-84831] -Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/antibody/nbp1-84831_rabbit-polyclonal-tfpi-antibody-immunohistochemistry-paraffin-29102024105638..png "Immunohistochemistry-Paraffin: TFPI Antibody [NBP1-84831] -Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: TFPI Antibody [NBP1-84831] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/antibody/nbp1-84831_rabbit-polyclonal-tfpi-antibody-immunohistochemistry-paraffin-29102024105732..png)
![Immunohistochemistry-Paraffin: TFPI Antibody [NBP1-84831] -Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-84831_rabbit-polyclonal-tfpi-antibody-immunohistochemistry-paraffin-29102024105827..png)
![Immunoprecipitation: TFPI Antibody (JE40-58) [NBP3-33007] - TFPI was immunoprecipitated from 0.2 mg HepG2 cell lysate with NBP3-33007 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33007 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HepG2 cell lysate (input)<br/> Lane 2: NBP3-33007 IP in HepG2 cell lysate<br/> Lane 3: Rabbit IgG instead of NBP3-33007 in HepG2 cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 52 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-33007_rabbit-tfpi-mab-je40-58-2682024158267.jpg "Immunoprecipitation: TFPI Antibody (JE40-58) [NBP3-33007] - TFPI was immunoprecipitated from 0.2 mg HepG2 cell lysate with NBP3-33007 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33007 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HepG2 cell lysate (input)<br/> Lane 2: NBP3-33007 IP in HepG2 cell lysate<br/> Lane 3: Rabbit IgG instead of NBP3-33007 in HepG2 cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 52 seconds; ECL")
![Immunohistochemistry: TFPI Antibody (JE40-58) [NBP3-33007] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-TFPI antibody (NBP3-33007) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33007) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33007_rabbit-tfpi-mab-je40-58-298202414161886.jpg)
![Western blot analysis on different lysates with NBP3-33007 at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: LoVo cell lysate (negative) Lane 3: HUVEC cell lysate Lane 4: EA.hy926 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 35-45 kDa Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33007 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33007_-western-blot-639204597489501124.jpg)
![Western Blot: TFPI Antibody [NB100-786] - Western blot analysis of TFPI in Hela lysate using NB100-786 at 0.05 ug/ ml.](http://images.novusbio.com/fullsize/TFPI-Antibody-Western-Blot-NB100-786-img0002.jpg "Western Blot: TFPI Antibody [NB100-786] - Western blot analysis of TFPI in Hela lysate using NB100-786 at 0.05 ug/ ml.")
![Western Blot: TFPI Antibody [NB100-786] - HEK293 overexpressing TFPI and probed with (mock transfection in first lane).](http://images.novusbio.com/fullsize/TFPI-Antibody-Western-Blot-NB100-786-img0001.jpg)
![Immunohistochemistry-Paraffin: TFPI Antibody (401) [NBP2-89570] - Staining of human TFPI in human kidney with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/TFPI-Antibody-401-Immunohistochemistry-Paraffin-NBP2-89570-img0001.jpg "Immunohistochemistry-Paraffin: TFPI Antibody (401) [NBP2-89570] - Staining of human TFPI in human kidney with rabbit monoclonal antibody (1:200).")
![Western Blot: TFPI Antibody (6) [NBP2-89569] - Lane A: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:40 kDa](http://images.novusbio.com/fullsize/TFPI-Antibody-6-Western-Blot-NBP2-89569-img0001.jpg "Western Blot: TFPI Antibody (6) [NBP2-89569] - Lane A: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:40 kDa")
![Immunoprecipitation: TFPI Antibody (6) [NBP2-89569] - Lane A:0.5 mg MCF-7 Whole Cell Lysate 0.5 uL anti-TFPI rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-TFPI rabbit monoclonal antibody,at 1:1000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size: 45 kDa](http://images.novusbio.com/fullsize/TFPI-Antibody-6-Immunoprecipitation-NBP2-89569-img0002.jpg)
![ELISA: TFPI Antibody (3A8) [NBP3-26290] - The Binding Activity of Rabbit TFPI with TFPI Antibody.<br /> Activity: Measured by its binding ability in a functional ELISA. Immobilized Rabbit TFPI at 1 ug/mL can bind TFPI Antibody, the EC50 is 2.281-3.783 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26290_human-tfpi-mab-3a8-262202412272319.jpg "ELISA: TFPI Antibody (3A8) [NBP3-26290] - The Binding Activity of Rabbit TFPI with TFPI Antibody.<br /> Activity: Measured by its binding ability in a functional ELISA. Immobilized Rabbit TFPI at 1 ug/mL can bind TFPI Antibody, the EC50 is 2.281-3.783 ng/mL.")
![ELISA: TFPI Antibody (3A8) [NBP3-26290] - The Binding Activity of Human TFPI with TFPI Antibody.<br /> Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TFPI at 1 ug/mL can bind TFPI Antibody, the EC50 is 1.242-1.788 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26290_human-tfpi-mab-3a8-262202412272322.jpg)
