TFPI Overexpression Lysate (Native)


Western Blot: TFPI Overexpression Lysate (Adult Normal) [NBL1-16845] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TFPI.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

TFPI Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC219033 and based on accession number NM_006287. The protein contains a C-terminal DDK tag.
Homo sapiens tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) (TFPI), transcript variant 1, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
31.9 kDa

Lysate Details for TFPI

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TFPI Overexpression Lysate (Native)

  • convertin
  • EPI
  • Extrinsic pathway inhibitor
  • LACI
  • Lipoprotein-associated coagulation inhibitor
  • tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
  • tissue factor pathway inhibitor


This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes isoform a precursor. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, PA
Species: Hu
Applications: WB, Simple Western
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Ba
Applications: WB, ELISA
Species: Hu
Applications: IHC
Species: Hu
Applications: WB, ELISA
Species: Hu
Applications: WB, IP, Neut

Publications for TFPI Lysate (NBL1-16845) (0)

There are no publications for TFPI Lysate (NBL1-16845).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TFPI Lysate (NBL1-16845) (0)

There are no reviews for TFPI Lysate (NBL1-16845). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TFPI Lysate (NBL1-16845) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TFPI Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for TFPI Lysate (NBL1-16845)

Discover related pathways, diseases and genes to TFPI Lysate (NBL1-16845). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TFPI Lysate (NBL1-16845)

Discover more about diseases related to TFPI Lysate (NBL1-16845).

Pathways for TFPI Lysate (NBL1-16845)

View related products by pathway.

PTMs for TFPI Lysate (NBL1-16845)

Learn more about PTMs related to TFPI Lysate (NBL1-16845).

Blogs on TFPI

There are no specific blogs for TFPI, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol TFPI

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-16845 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

Plasminogen Antibody
Plasminogen Antibody
Plasminogen Antibody