TET3 Antibody

Western Blot: TET3 Antibody [NBP2-20602] - BMMSCs express Tet1, Tet2, and Tet3. Both human (h) and mouse (m) BMMSCs expressed Tet1, Tet2, and Tet3, as assessed by western blotting. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04464-6) licensed under a CC-BY license.

Immunoprecipitation: TET3 Antibody [NBP2-20602] - Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 ug of TET3 antibody 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.

Immunocytochemistry/ Immunofluorescence: TET3 Antibody [NBP2-20602] - BMMSCs express Tet1, Tet2, & Tet3. a, b Both human (h) & mouse (m) BMMSCs expressed Tet1, Tet2, & Tet3, as assessed by western blotting (a) & qPCR (b). c Immunocytofluorescent staining showed that CD146-positive BMMSCs expressed Tet1, Tet2, & Tet3. Scale bar, 50 μm. Results are from three independent experiments. ***p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TET3 protein stained by TET3 antibody [C3], C-term (NBP2-20602) diluted at 1:400.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

• Reactivity: Hu, Mu, Rt, Bv, Xp
• Applications: WB, Flow, ICC/IF, IHC, IP, ChIP, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TET3 Antibody Summary

• Description: Novus Biologicals Rabbit TET3 Antibody (NBP2-20602) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF, IP and ChIP. Anti-TET3 Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TET3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) Assay dependent
  • Flow Cytometry Reported in scientific literature (PMID:34246869)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry Whole-Mount Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:500-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 179 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34246869)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TET3 Antibody

• EC 1.14.11.n2
• hCG_40738
• KIAA0401
• methylcytosine dioxygenase TET3
• MGC22014
• probable methylcytosine dioxygenase TET3
• tet oncogene family member 3

Background

Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TET3 Antibody (NBP2-20602)(12)

Publications using NBP2-20602

• Parik Kakani, Shruti Ganesh Dhamdhere, Deepak Pant, Rushikesh Joshi, Jharna Mishra, Atul Samaiya, Sanjeev Shukla Hypoxia-induced CTCF promotes EMT in breast cancer. Cell reports 2024-06-18 [PMID: 38900639]
• Zhang K, Lan T, Lin F et al. Effect of vitrification on protein O-GlcNAcylation in mouse metaphase ? oocytes Reproduction (Cambridge, England) 2023-10-01 [PMID: 37903183]
• Liu X, Cui H The palliative effects of folic acid on retinal microvessels in diabetic retinopathy via regulating the metabolism of DNA methylation and hydroxymethylation Bioengineered 2021-12-01 [PMID: 34874218] (Chemotaxis, Rat)
• Deshpande Sss, Nemani H, Balasinor Nh High fat diet-induced- and genetically inherited- obesity differential alters DNA demethylation pathways in the germline of adult male rats Reproductive biology 2021-07-08 [PMID: 34246869] (FLOW)
• Wu SL, Zhang X, Chang M et al. Genome-wide 5-hydroxymethylcytosine Profiling Analysis Identifies MAP7D1 as A Novel Regulator of Lymph Node Metastasis in Breast Cancer Genomics, proteomics & bioinformatics 2021-03-11 [PMID: 33716151] (IF/IHC, Human)
• Jia M, Li Z, Pan M et al. LINC-PINT suppresses aggressiveness of thyroid cancer by downregulating miR-767-5p to induce TET2 expression Mol Ther Nucleic Acids 2020-11-24 [PMID: 33230437]
• Atlante S, Visintin A, Marini E et al. a-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis. Cell Death Dis 2018-07-09 [PMID: 29988033] (WB, Mouse)
• Yang R, Yu T, Kou X et al. Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter Nat Commun 2018-06-01 [PMID: 29858571] (WB, Mouse, Human)
• Ma M, Zhou QJ, Xiong Y, Li B. Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1. Am J Transl Res. 2018-01-15 [PMID: 29422991] (IHC-P, Human)
• Le Bras A, Yu B, Issa Bhaloo S et al. Adventitial Sca1+ Cells Transduced With ETV2 Are Committed to the Endothelial Fate and Improve Vascular Remodeling After Injury Arterioscler. Thromb. Vasc. Biol. [PMID: 29191922] (ICC/IF, Mouse)
• Gao J, Wang H, Shen J et al. Mutual regulation between GDF11 and TET2 prevents senescence of mesenchymal stem cells bioRxiv 2020-01-01 (WB, Human)
• Cao D, Jia Z, Wu Y et al. Demethylation of the RB1 promoter concomitant with reactivation of TET2 and TET3 impairs gastric carcinogenesis in K19-Wnt1/C2mE transgenic mice Life Sci 2020-10-12 [PMID: 33058920] (WB, IHC-P, Human, Mouse)

Review for TET3 Antibody (NBP2-20602) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 10/03/2014

Summary

• Application: Western Blot
• Sample Tested: MOUSE FIBROBLAST
• Species: Mouse
• Lot: A1

Bioinformatics

• Gene Symbol: TET3
• Uniprot:
  • O43151(Human)