TET3 Antibody

Images

 
Western Blot: TET3 Antibody [NBP2-20602] - BMMSCs express Tet1, Tet2, and Tet3. Both human (h) and mouse (m) BMMSCs expressed Tet1, Tet2, and Tet3, as assessed by western blotting. Image collected and cropped by CiteAb ...read more
Immunocytochemistry/ Immunofluorescence: TET3 Antibody [NBP2-20602] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET3 protein stained by TET3 antibody [C3], C-term diluted at 1:400. Red: ...read more
Immunohistochemistry-Paraffin: TET3 Antibody [NBP2-20602] - Mouse colon. TET3 stained by TET3 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min.
Western Blot: TET3 Antibody [NBP2-20602] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term diluted ...read more
Immunoprecipitation: TET3 Antibody [NBP2-20602] - Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune rabbit IgG C. ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, IHC-WhMt
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TET3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TET3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10-1:500
  • Immunohistochemistry Whole-Mount 1:10 - 1:500
Theoretical MW
179 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-20602 in the following applications:

Publications
Read Publications using
NBP2-20602 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for TET3 Antibody

  • EC 1.14.11.n2
  • hCG_40738
  • KIAA0401
  • methylcytosine dioxygenase TET3
  • MGC22014
  • probable methylcytosine dioxygenase TET3
  • tet oncogene family member 3

Background

Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TET3 Antibody (NBP2-20602)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


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ICC/IF
(1)
IHC-P
(2)
WB
(5)
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Human
(5)
Mouse
(4)
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Showing Publications 1 - 7 of 7.
Publications using NBP2-20602 Applications Species
Gao J, Wang H, Shen J et al. Mutual regulation between GDF11 and TET2 prevents senescence of mesenchymal stem cells bioRxiv Jan 1 2020 (WB, Human) WB Human
Jia M, Li Z, Pan M et al. LINC-PINT suppresses aggressiveness of thyroid cancer by downregulating miR-767-5p to induce TET2 expression Molecular Therapy - Nucleic Acids Jun 1 2020 (WB, Human) WB Human
Cao D, Jia Z, Wu Y et al. Demethylation of the RB1 promoter concomitant with reactivation of TET2 and TET3 impairs gastric carcinogenesis in K19-Wnt1/C2mE transgenic mice Life Sci Oct 12 2020 [PMID: 33058920] (WB, IHC-P, Human, Mouse) WB, IHC-P Human, Mouse
Atlante S, Visintin A, Marini E et al. a-ketoglutarate dehydrogenase inhibition counteracts breast cancer-associated lung metastasis. Cell Death Dis Jul 9 2018 [PMID: 29988033] (WB, Mouse) WB Mouse
Yang R, Yu T, Kou X et al. Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter Nat Commun Jun 1 2018 [PMID: 29858571] (WB, Human, Mouse) WB Human, Mouse
Ma M, Zhou QJ, Xiong Y, Li B. Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1. Am J Transl Res. 2018 Jan 15 [PMID: 29422991] (IHC-P, Human) IHC-P Human
Le Bras A, Yu B, Issa Bhaloo S et al. Adventitial Sca1+ Cells Transduced With ETV2 Are Committed to the Endothelial Fate and Improve Vascular Remodeling After Injury Arterioscler. Thromb. Vasc. Biol. [PMID: 29191922] (ICC/IF, Mouse) ICC/IF Mouse

Review for TET3 Antibody (NBP2-20602) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-20602:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot TET3 NBP2-20602
Enlarge
4
reviewed by:
Anonymous
WB Mouse 10/03/2014
View

Summary

ApplicationWestern Blot
Sample TestedMOUSE FIBROBLAST
SpeciesMouse
LotA1
CommentsThis product, since it is a polyclonal one gives a lot of background / non-specific bindings, it is difficult to identify the original band.

Secondary Antibody

Secondary Descriptionanti-rabbit HRP
Secondary Concentration1:1000

Comments

CommentsThis product, since it is a polyclonal one gives a lot of background / non-specific bindings, it is difficult to identify the original band.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TET3 Antibody (NBP2-20602) (0)

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Secondary Antibodies

 

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Recent Reviews

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5
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4
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1
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Anonymous
10/03/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TET3
Uniprot