TET3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: TET3 Antibody [NBP2-13427] - Staining of human cell line MCF7 shows localization to nucleoplasm, cytosol & vesicles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TET3 Antibody [NBP2-13427] - Staining of human uterine cervix shows moderate nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: TET3 Antibody [NBP2-13427] - Staining of human duodenum shows weak nuclear positivity in a subset of glandular cells.

Immunohistochemistry-Paraffin: TET3 Antibody [NBP2-13427] - Staining of human placenta shows moderate nuclear positivity in a subset of cells in chorionic villi.

Immunohistochemistry-Paraffin: TET3 Antibody [NBP2-13427] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TET3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TET3 Antibody - BSA Free (NBP2-13427) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-TET3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: SLKGGLSQQGLKPSLKVEPQNHFSSFKYSGNAVVESYSVLGNCRPSDPYSMNSVYSYHSYYAQPSLTSVNGFHSKYALPSFSYYGFPSSNPVFPSQFLGPGAWGHSGSSGSFEKKPDLH
• Predicted Species: Mouse (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TET3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TET3 Protein (NBP2-13427PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TET3 Antibody - BSA Free

• EC 1.14.11.n2
• hCG_40738
• KIAA0401
• methylcytosine dioxygenase TET3
• MGC22014
• probable methylcytosine dioxygenase TET3
• tet oncogene family member 3

Background

TET3 may catalyze the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TET3 Antibody (NBP2-13427)(1)

Publication using NBP2-13427

• Ni K, Dansranjavin T, Rogenhofer N et al. TET enzymes are successively expressed during human spermatogenesis and their expression level is pivotal for male fertility. Hum Reprod 2016-07-01 [PMID: 27141042]

Bioinformatics

• Gene Symbol: TET3