Tensin 4/CTEN Antibody (684524)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Tensin 4/CTEN Monoclonal Antibody (Catalog # MAB6925) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tensin 4/CTEN at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Tensin 4/CTEN was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Tensin 4/ CTEN Monoclonal Antibody (Catalog # MAB6925) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Tensin 4/CTEN at approximately 74 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Tensin 4/CTEN Monoclonal Antibody (Catalog # MAB6925). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clone: 684524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Tensin 4/CTEN Antibody (684524) Summary

• Immunogen: E. coli-derived recombinant human Tensin 4/CTEN
  Ser2-Gln107
  Accession # Q8IZW8
• Specificity: Detects human Tensin 4/CTEN in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNS4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tensin 4/CTEN Antibody (684524)

• CTEN
• CTENC terminal tensin like
• C-terminal tensin-like protein
• FLJ14950
• Tensin 4
• tensin-4
• TNS4

Background

CTEN, also known as Tensin 4, is a 90 kDa cytoplasmic protein that is associated with focal adhesions in epithelial cells and is upregulated in several carcinomas. It promotes tumorigenesis by disrupting the attachment of focal adhesions to the cytoskeleton, promoting epithelial-mesenchymal transition, and binding nuclear beta‑Catenin. CTEN is cleaved by Caspase-3 at Asp570-Ser571 during apoptosis. It contains a serine-rich region (aa 168-324), one SH2 domain (aa 449-556), and a phosphatase domain (aa 548-714). Within aa 1-107, human CTEN shares 62% and 59% aa sequence identity with mouse and rat CTEN, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tensin 4/CTEN Antibody (MAB6925)(2)

Publications using MAB6925

• JC Fleming, J Woo, K Moutasim, CJ Hanley, SJ Frampton, O Wood, M Ward, CH Woelk, CH Ottensmeie, S Hafizi, D Kim, GJ Thomas CTEN Induces Tumour Cell Invasion and Survival and Is Prognostic in Radiotherapy-Treated Head and Neck Cancer Cancers (Basel), 2020-10-13;12(10):. 2020-10-13 [PMID: 33066224] (IHC, Human)
• Sjoestroem C, Khosravi S, Zhang G, Martinka M, Li G C-terminal tensin-like protein is a novel prognostic marker for primary melanoma patients. PLoS ONE, 2013-11-07;8(11):e80492. 2013-11-07 [PMID: 24244691] (IHC-P, Human)

Bioinformatics

• Gene Symbol: TNS4
• Uniprot:
  • Q8IZW8()