TEM8/ANTXR1 Antibody - BSA Free

Western Blot: TEM8/ANTXR1 Antibody [NBP1-77233] - HepG2 cell lysates with ATR antibody (IN) at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunohistochemistry: TEM8/ANTXR1 Antibody - BSA Free [NBP1-77233] - Immunohistochemical staining of human brain tissue using TEM8/ANTXR1 antibody at 2 u/mL.

Immunocytochemistry/ Immunofluorescence: TEM8/ANTXR1 Antibody - BSA Free [NBP1-77233] - Immunofluorescence of TEM8/ANTXR1 in Human Brain tissue with TEM8/ANTXR1 antibody at 10 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TEM8/ANTXR1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 13 amino acids near the center of human ATR. The immunogen is located within amino acids 220 - 270 of ATR. Amino Acid Squence: RGNGFRHARNVDR
• Specificity: Recognizes all three isoforms of ATR.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANTXR1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 0.5-2 ug/ml
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)
  HepG2 Whole Cell Lysate

• Control Peptide:

  TEM8/ANTXR1 Protein (NBP1-77233PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TEM8/ANTXR1 Antibody - BSA Free

• anthrax toxin receptor 1
• ANTXR1
• ATR2310008J16Rik
• FLJ10601
• FLJ11298
• FLJ21776
• TEM8
• TEM82810405N18Rik
• Tumor endothelial marker 8

Background

The Anthrax toxin receptor (ATR) was initially discovered as the tumor endothelial marker 8 (TEM8). This protein, which exists in three isoforms (36, 40, and 60 kDa), is highly expressed in tumor vessels as well as in the vasculature of developing embryos, suggesting that it may normally play a role in angiogenesis. However, it also acts as the receptor for anthrax toxin. Following the binding of this protein by the protective antigen (PA) of anthrax, PA is cleaved and heptamerizes to form the binding site for both edema factor (EF) and lethal factor (LF). This complex is then endocytosed by the cell; acidification in endosomes allows the release of EF and LF into the cytoplasm where they interfere with MAPK signaling and induce apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANTXR1
• Entrez:
  • 84168(Human)
  • 69538(Mouse)
  • 362393(Rat)
• Uniprot:
  • Q9H6X2()