CMG-2/ANTXR2 Antibody [Unconjugated]

Images

 
Human whole blood monocytes were stained with Human CMG‑2/ANTXR2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2940, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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CMG-2/ANTXR2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CMG‑2/ANTXR2 isoform 1
Gln34-Asn317
Accession # P58335
Specificity
Detects human CMG‑2/ANTXR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse CMG-2 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ANTXR2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • ELISA
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
Publications
Read Publications using
AF2940 in the following applications:

  • IP
    1 publication
  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CMG-2/ANTXR2 Antibody [Unconjugated]

  • anthrax toxin receptor 2
  • ANTXR2
  • Capillary morphogenesis gene 2 protein
  • capillary morphogenesis protein 2
  • cI-35
  • CMG2
  • CMG-2
  • CMG2MGC111533
  • CMG-2MGC45856
  • FLJ31074
  • ISH
  • JHF
  • JHS

Background

Capillary Morphogenesis Gene-2 (CMG-2) is a widely expressed anthrax toxin receptor (ATR) family protein (1‑3). CMG-2 is a 55 kDa type I transmembrane (TM) protein that contains a 33 amino acid (aa) signal sequence, a 284 aa extracellular domain (ECD), a 24 aa TM segment, and a 147 aa cytoplasmic domain. There are three additional isoforms. Isoforms 4 shows a 12 aa insertion in the cytoplasmic region; isoform 2 shows a 103 aa deletion in the ECD; and isoform 3 is a truncated, 20 kDa, 289 aa soluble form. The main functional domain of CMG-2 is an extracellular integrin-like von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site (MIDAS). This domain adheres selectively to collagen type IV and laminin (1‑5). CMG-2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (3). CMG-2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (6). CMG-2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus Anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore and binds LF or EF toxin subunits (5, 7, 8). Transport to low pH endosomes, which requires CMG-2 ubiquitination and interaction with the LDL receptor related protein LRP6 (9, 10), allows PA pore formation and release of toxin to the cytoplasm (10, 11). Soluble CMG-2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). Within the extracellular region, human CMG-2 shares 84%, 81%,89% and 93% amino acid sequence homology with mouse, rat, bovine, and canine CMG-2, respectively. CMG-2 VWA domain also shares 60% aa identity with ATR/TEM8.
  1. Scobie, H.M. and J.A.T. Young (2005) Curr. Opin. Microbiol. 8:106.
  2. Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.
  3. Bell, S.E. et al. (2001) J. Cell Sci. 114:2755.
  4. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367.
  5. Santelli, E. et al. (2004) Nature 430:905.
  6. Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957.
  7. Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349.
  8. Go, M.Y. et al. (2006) J. Mol. Biol. 360:145.
  9. Abrami, L. et al. (2006) J. Cell Biol. 172:309.
  10. Wei, W. et al. (2006) Cell 124:1141.
  11. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CMG-2/ANTXR2 Antibody (AF2940)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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(1)
WB
(4)
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(5)
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Showing Publications 1 - 5 of 5.
Publications using AF2940 Applications Species
Friebe S, Deuquet J, van der Goot F Differential dependence on N-glycosylation of anthrax toxin receptors CMG2 and TEM8. PLoS ONE, 2015;10(3):e0119864. 2015 [PMID: 25781883] (IP,, Human) IP Human
Ye L, Sun P, Sanders A, Martin T, Lane J, Mason M, Jiang W Therapeutic potential of capillary morphogenesis gene 2 extracellular vWA domain in tumourrelated angiogenesis. Int J Oncol, 2014;45(4):1565-73. 2014 [PMID: 24993339] (WB,, Human) WB Human
Ye, Lin, Sanders, Andrew J, Sun, Ping-Hui, Mason, Malcolm, Jiang, Wen G Capillary morphogenesis gene 2 regulates adhesion and invasiveness of prostate cancer cells. Oncol Lett, 2014;7(6):2149-2153. 2014 [PMID: 24932305] (WB,, Human) WB Human
Langer, Marybeth, Duggan, Elizabet, Booth, John Lel, Patel, Vineet I, Zander, Ryan A, Silasi-Mansat, Robert, Ramani, Vijay, Veres, Tibor Zo, Prenzler, Frauke, Sewald, Katherin, Williams, Daniel M, Coggeshall, Kenneth, Awasthi, Shanjana, Lupu, Florea, Burian, Dennis, Ballard, Jimmy Da, Braun, Armin, Metcalf, Jordan P Bacillus anthracis lethal toxin reduces human alveolar epithelial barrier function. Infect Immun, 2012;80(12):4374-87. 2012 [PMID: 23027535] (WB,, Human) WB Human
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J. Immunol., 2009;183(9):5799-806. 2009 [PMID: 19812208] (WB,, Human) WB Human

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Bioinformatics

Gene Symbol ANTXR2
Entrez
Uniprot