ATRIP Antibody (OTI5E7)

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Western Blot: ATRIP Antibody (OTI5E7) [NBP2-02004] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-ATRIP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunocytochemistry/ Immunofluorescence: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATRIP.
Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human tonsil using anti-ATRIP mouse monoclonal antibody.
Flow Cytometry: ATRIP Antibody (OTI5E7) [NBP2-02004] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATRIP antibody, and then ...read more
Western Blot: ATRIP Antibody (OTI5E7) [NBP2-02004] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATRIP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATRIP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATRIP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human breast tissue using anti-ATRIP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human Kidney tissue using anti-ATRIP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human liver tissue using anti-ATRIP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human pancreas tissue using anti-ATRIP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ATRIP Antibody (OTI5E7) [NBP2-02004] - Staining of paraffin-embedded Human prostate tissue using anti-ATRIP mouse monoclonal antibody.
Flow Cytometry: ATRIP Antibody (OTI5E7) [NBP2-02004] - Analysis of Hela cells, using anti-ATRIP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: ATRIP Antibody (OTI5E7) [NBP2-02004] - Analysis of Jurkat cells, using anti-ATRIP antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI5E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ATRIP Antibody (OTI5E7) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 42-309 of human ATRIP(NP_569055) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ATRIP
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
85.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for ATRIP Antibody (OTI5E7)

  • AGS1
  • ATM and Rad3-related-interacting protein
  • ATR interacting protein
  • ATR-interacting protein
  • ATRIP
  • DKFZp762J2115
  • FLJ12343
  • MGC20625
  • MGC21482
  • MGC26740
  • TREX1

Background

The product of this gene is an essential component of the DNA damage checkpoint, and binds to single-stranded DNA coated with replication protein A that accumulates at sites of DNA damage. The encoded protein interacts with the ataxia telangiectasia and Rad3 related protein, a checkpoint kinase, resulting in accumulation of the kinase at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATRIP Antibody (NBP2-02004) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-72271AF350
Alexa Fluor 405 NBP2-72271AF405
Alexa Fluor 488 NBP2-72271AF488
Alexa Fluor 532 NBP2-72271AF532
Alexa Fluor 594 NBP2-72271AF594
Alexa Fluor 647 NBP2-72271AF647
Alexa Fluor 700 NBP2-72271AF700
Alexa Fluor 750 NBP2-72271AF750
Allophycocyanin NBP2-72271APC
Allophycocyanin/Cy7 NBP2-72271APCCY7
Biotin NBP2-72271B
DyLight 350 NBP2-72271UV
DyLight 405 NBP2-72271V
DyLight 488 NBP2-72271G
DyLight 550 NBP2-72271R
DyLight 594 NBP2-72271DL594
DyLight 650 NBP2-72271C
DyLight 680 NBP2-72271FR
DyLight 755 NBP2-72271IR
FITC NBP2-72271F
HRP NBP2-72271H
Janelia Fluor 549 NBP2-72271JF549
Janelia Fluor 646 NBP2-72271JF646
PE NBP2-72271PE
PE/Atto594 NBP2-72271PEATT594
PE/Cy5.5 NBP2-72271PECY55
PE/Cy7 NBP2-72271PECY7
PerCP NBP2-72271PCP

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Bioinformatics

Gene Symbol ATRIP
Entrez