TEM7/PLXDC1 Antibody (197C193 (IM193)) - Azide and BSA Free

Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193)) - Azide Free [NBP2-33228] - Analysis of human breast cancer lysate (35 ug per lane, RIPA buffer) at 0.03ug/ml. Band observed at ~60kDa. (Expected MW of 55.8kDa according to NP_065138.2).

Immunohistochemistry: TEM7/PLXDC1 Antibody (197C193 (IM193)) - Azide Free [NBP2-33228] - Formalin-fixed, paraffin-embedded human breast vessel stained with TEM7 antibody at 5 ug/ml.

Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193)) - Azide Free [NBP2-33228] - Analysis in human HCT-116 cell lysate at 1 ug/ml.

Immunohistochemistry: TEM7/PLXDC1 Antibody (197C193 (IM193)) - Azide Free [NBP2-33228] - Staining of Tumor Endothelial Marker 7 in formalin-fixed, paraffin-embedded human Purkinje neurons at 2.5 ug/ml. Hematoxylin-eosin counterstain.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: 197C193 (IM193)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

TEM7/PLXDC1 Antibody (197C193 (IM193)) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TEM7/PLXDC1 Antibody (197C193 (IM193)) - Azide and BSA Free (NB100-56557) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Amino acids 409-425 (LQNNLSPKTKGTPVHLG) of human TEM7 were used to develop this monoclonal antibody.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLXDC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500. Use reported in scientific literature (Meng et al (2007))
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500. Use reported in scientific literature (Lee et al (2006))
  • Immunohistochemistry-Paraffin 2 - 5 ug/ml. Use 2-5 ug/ml
  • Immunoprecipitation reported in scientific literature (Nanda et al (2004))
  • Western Blot 1-3 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TEM7/PLXDC1 Antibody (197C193 (IM193)) - Azide and BSA Free

• FLJ45632
• plexin domain containing 1,2410003I07Rik
• plexin domain-containing protein 1
• PLXDC1
• TEM3
• TEM3FLJ36270
• TEM7
• TEM7DKFZp686F0937
• Tumor endothelial marker 3
• Tumor endothelial marker 7

Background

Recently, using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al, have identified 46 genes, whose expression is specifically elevated in tumor-associated endothelium. Nine of these genes were prominently expressed only in tumor endothelial cells (EC), but were absent or barely detectable in normal ECs, and named as Tumor Endothelial Markers (TEMs, TEM 1-9). TEM7 (Tumor endothelial marker 7) transcripts are specifically expressed in the endothelium of colorectal cancer, primary cancers of lung, pancreas, breast, and brain. TEM7 is expressed specifically in endothelium of these cancers, whether primary or metastasis. The other six members of this family (TEM1, 3, 4, 5, 8, and 9) also show similar expression pattern in lung and brain tumors, and liver metastasis. Since most of the genes expressed differentially in tumor endothelium are also expressed during angiogenesis, these newly discovered genes might provide important resources for basic and clinical studies of human angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLXDC1