Apc10 Antibody (8F1D10)

Images

 
Western Blot: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis using ANAPC10 mAb against HEK293 (1) and ANAPC10 (AA: 1-185)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of Hela cells using ANAPC10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of paraffin-embedded esophageal cancer tissues using ANAPC10 mouse mAb with DAB staining.
Flow Cytometry: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of Hela cells using ANAPC10 mouse mAb (green) and negative control (red).
Western Blot: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis using ANAPC10 mAb against human ANAPC10 (AA: 1-185) recombinant protein. (Expected MW is 47.2 kDa)
Western Blot: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis using ANAPC10 mouse mAb against Hela (1), MCF-7 (2), SK-Br-3 (3), A431 (4), HEK293 (5), A549 (6), and SPC-A-1 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of Hela cells using ANAPC10 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- ...read more
Immunohistochemistry: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of paraffin-embedded colon cancer tissues using ANAPC10 mouse mAb with DAB staining.
ELISA: Apc10 Antibody (8F1D10) [NBP2-61889] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
8F1D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Apc10 Antibody (8F1D10) Summary

Immunogen
Purified recombinant fragment of human ANAPC10 (AA: 1-185) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ANAPC10
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
Theoretical MW
46.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Apc10 Antibody (8F1D10)

  • anaphase promoting complex subunit 10
  • anaphase-promoting complex subunit 10
  • APC10DOC1
  • Cyclosome subunit 10
  • DKFZP564L0562

Background

ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-66987
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89095
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89093
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-20287
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-77375
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-P
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-57669
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-61656
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-01128
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF4497
Species: Hu
Applications: WB, Simple Western
NBP2-21037
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-15840
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-20389
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NB100-59828
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
AF2574
Species: Hu
Applications: CyTOF-ready, ICFlow
NBP2-31363
Species: Hu
Applications: WB, ICC/IF
NBP2-61889
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC

Publications for Apc10 Antibody (NBP2-61889) (0)

There are no publications for Apc10 Antibody (NBP2-61889).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Apc10 Antibody (NBP2-61889) (0)

There are no reviews for Apc10 Antibody (NBP2-61889). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Apc10 Antibody (NBP2-61889) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Apc10 Antibody (NBP2-61889)

Discover related pathways, diseases and genes to Apc10 Antibody (NBP2-61889). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Apc10 Antibody (NBP2-61889)

Discover more about diseases related to Apc10 Antibody (NBP2-61889).
 

Pathways for Apc10 Antibody (NBP2-61889)

View related products by pathway.

Research Areas for Apc10 Antibody (NBP2-61889)

Find related products by research area.

Blogs on Apc10

There are no specific blogs for Apc10, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Apc10 Antibody (8F1D10) and receive a gift card or discount.

Bioinformatics

Gene Symbol ANAPC10