Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: TEM-1 4357, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: ...read more
Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Human colon tissue with TEM1 antibody at 2.5 ug/ml.
Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Analysis of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 ug/mL.
Independent Antibodies: Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: ...read more
Immunofluorescence: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Human Colon tissue with TEM1 antibody at 20 ug/mL.
Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human TEM1. The immunogen is located within the last 50 amino acids of TEM1.
Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD) and isoform 2 (433aa, 46kD). Mouse TEM-1 has one isoform (765aa, 82kD) and rat TEM-1 also has one isoform (764aa, 82kD). NBP1-77310 can detect all isoforms of human, mouse and rat.
Peptide affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use in ICC/IF with Human samples reported in scientific literature (PMID: 30285896). Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
46, 81 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP1-77310 in the following applications:
Expected species cross reactivity based on sequence homology: Mouse (86%), Rat (86%)
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified
Alternate Names for Endosialin/CD248/TEM1 Antibody
CD164 sialomucin-like 1
CD248 antigen, endosialin
CD248 molecule, endosialin
Tumor endothelial marker 1
Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion. At least two isoforms of TEM1 are known to exist; this antibody recognizes both isoforms.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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