Endosialin/CD248/TEM1 Antibody

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Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: TEM-1 4357, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: ...read more
Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Human colon tissue with TEM1 antibody at 2.5 ug/ml.
Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Analysis of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 ug/mL.
Independent Antibodies: Western Blot: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: ...read more
Immunofluorescence: Endosialin/CD248/TEM1 Antibody [NBP1-77310] - Human Colon tissue with TEM1 antibody at 20 ug/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

Endosialin/CD248/TEM1 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human TEM1. The immunogen is located within the last 50 amino acids of TEM1.
Specificity
Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD) and isoform 2 (433aa, 46kD). Mouse TEM-1 has one isoform (765aa, 82kD) and rat TEM-1 also has one isoform (764aa, 82kD). NBP1-77310 can detect all isoforms of human, mouse and rat.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD248
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Immunofluorescence 20 ug/ml
Application Notes
Use in ICC/IF with Human samples reported in scientific literature (PMID: 30285896). Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Theoretical MW
46, 81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Publications
Read Publication using
NBP1-77310 in the following applications:

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (86%), Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Endosialin/CD248/TEM1 Antibody

  • 2610111G01Rik
  • CD164 sialomucin-like 1
  • CD164L1
  • CD248 antigen
  • CD248 antigen, endosialin
  • CD248 molecule, endosialin
  • CD248
  • Endosialin
  • MGC119479
  • TEM1
  • TEM1MGC119478
  • Tumor endothelial marker 1

Background

Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion. At least two isoforms of TEM1 are known to exist; this antibody recognizes both isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endosialin/CD248/TEM1 Antibody (NBP1-77310)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CD248
Entrez
Uniprot