TEM7/PLXDC1 Antibody (197C193 (IM193)) - BSA Free

Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Analysis of human breast cancer lysate (35 ug per lane, RIPA buffer) at 0.03ug/ml. Band observed at ~60kDa. (Expected MW of 55.8kDa according to NP_065138.2).

Immunohistochemistry: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Staining of Tumor Endothelial Marker 7 in formalin-fixed, paraffin-embedded human Purkinje neurons at 2.5 ug/ml. Hematoxylin-eosin counterstain.

Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Detection of TEM7 with TEM7 antibody. Human HCT-116 cell lysate probed with TEM7 antibody at 1 ug/ml.

Immunohistochemistry-Paraffin: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Formalin-fixed, paraffin-embedded human breast vessel stained with TEM7 antibody at 5 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: 197C193 (IM193)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TEM7/PLXDC1 Antibody (197C193 (IM193)) - BSA Free Summary

• Immunogen: Amino acids 409-425 (LQNNLSPKTKGTPVHLG) of human TEM7 were used to develop this monoclonal antibody.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLXDC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500. Use reported in scientific literature (Meng et al (2007))
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen reported in scientific literature (Lee et al (2006))
  • Immunohistochemistry-Paraffin 2-5 ug/ml
  • Immunoprecipitation 1:10-1:500. Use reported in scientific literature (Nanda et al (2004))
  • Western Blot
• Application Notes: Immunohistochemistry-Paraffin reported in scientific literature (Nanda et al (2004); Lee et al (2005))
• Control:

  TEM7/PLXDC1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TEM7/PLXDC1 Antibody (197C193 (IM193)) - BSA Free

• FLJ45632
• plexin domain containing 1,2410003I07Rik
• plexin domain-containing protein 1
• PLXDC1
• TEM3
• TEM3FLJ36270
• TEM7
• TEM7DKFZp686F0937
• Tumor endothelial marker 3
• Tumor endothelial marker 7

Background

Recently, using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al, have identified 46 genes, whose expression is specifically elevated in tumor-associated endothelium. Nine of these genes were prominently expressed only in tumor endothelial cells (EC), but were absent or barely detectable in normal ECs, and named as Tumor Endothelial Markers (TEMs, TEM 1-9). TEM7 (Tumor endothelial marker 7) transcripts are specifically expressed in the endothelium of colorectal cancer, primary cancers of lung, pancreas, breast, and brain. TEM7 is expressed specifically in endothelium of these cancers, whether primary or metastasis. The other six members of this family (TEM1, 3, 4, 5, 8, and 9) also show similar expression pattern in lung and brain tumors, and liver metastasis. Since most of the genes expressed differentially in tumor endothelium are also expressed during angiogenesis, these newly discovered genes might provide important resources for basic and clinical studies of human angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TEM7/PLXDC1 Antibody (NB100-56557)(9)

Publications using NB100-56557

• M Balzano, M De Grandis, TP Vu Manh, L Chasson, F Bardin, A Farina, A Sergé, G Bidaut, P Charbord, L Hérault, AL Bailly, A Cartier-Mi, A Boned, M Dalod, E Duprez, P Genever, M Coles, M Bajenoff, L Xerri, M Aurrand-Li, C Schiff, SJC Mancini Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche Cell Rep, 2019-03-19;26(12):3257-3271.e8. 2019-03-19 [PMID: 30893599]
• Carpenter RL, Paw I, Zhu H et al. The gain-of-function GLI1 transcription factor TGLI1 enhances expression of VEGF-C and TEM7 to promote glioblastoma angiogenesis. Oncotarget 2015-09-08 [PMID: 26093087] (IF/IHC)
• Mehran R, Nilsson M, Khajavi M et al. Tumor endothelial markers define novel subsets of cancer-specific circulating endothelial cells associated with antitumor efficacy. Cancer Res. 2014-03-13 [PMID: 24626092] (IHC-Fr, Mouse, Human)
  
  Details:
  Mouse zenograft tumor models with human and mouse tumor cells & normal mouse lung, Fig 1.
• Yamaji Y, Yoshida S, Ishikawa K et al. TEM7 (PLXDC1) in neovascular endothelial cells of fibrovascular membranes from patients with proliferative diabetic retinopathy. Invest Ophthalmol Vis Sci. 2008-07-01 [PMID: 18316703] (IHC-P, Human)
  
  Details:
  IHC (paraffin), human fibrovascular membranes, Figs. 3, 4, 5.
• Fuchs Bruno, Mahlum Eric, Halder Chandralekha et al. High expression of tumor endothelial marker 7 is associated with metastasis and poor survival of patients with osteogenic sarcoma. Gene. 2007-09-15 [PMID: 17560052] (Human)
• Meng F, Henson R, Patel T. Chemotherapeutic stress selectively activates NF-kappa B-dependent AKT and VEGF expression in liver cancer-derived endothelial cells. Am J Physiol Cell Physiol. 2007-08-01 [PMID: 17537803] (WB, ICC/IF)
  
  Details:
  WB (Fig 1A): AML12, normal mouse liver hepatocytes, and Hepa 1-6 mouse liver hepatoma cell lines, and mouse liver endothelial cells IF/ICC (Fig 1B): mouse liver endothelial cell monolayers.
• Lee HK, Kang DS, Seo IA et al. Expression of tumor endothelial marker 7 mRNA and protein in the dorsal root ganglion neurons of the rat. Neurosci Lett. 2006-07-10 [PMID: 16707219] (IHC-Fr)
• Lee HK, Bae HR, Park HK et al. Cloning, characterization and neuronal expression profiles of tumor endothelial marker 7 in the rat brain. Brain Res Mol Brain Res. 2005-05-20 [PMID: 15893603] (IHC-P)
• Nanda A, Buckhaults P, Seaman S et al. Identification of a binding partner for the endothelial cell surface proteins TEM7 and TEM7R. Cancer Res. 2004-12-01 [PMID: 15574754] (WB, Human)
  
  Details:
  WB: TEM7 transfected 293 cells (Figs 1C and 3B); human colon cancer and normal colon tissue lysates (Fig 1D), IP: TEM7 tranfected 293 cells (Figs 1C and 3B); IHC-paraffin: normal/tumor colon, esophagus and lung tissues (Fig 2A).

Bioinformatics

• Gene Symbol: PLXDC1
• Entrez:
  • 57125(Human)
• Uniprot:
  • Q8IUK5(Human)