TEM7/PLXDC1 Antibody (197C193 (IM193))

Images

 
Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Analysis of human breast cancer lysate (35 ug per lane, RIPA buffer) at 0.03ug/ml. Band observed at ~60kDa. (Expected MW of 55.8kDa according to ...read more
Immunohistochemistry: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Staining of Tumor Endothelial Marker 7 in formalin-fixed, paraffin-embedded human Purkinje neurons at 2.5 ug/ml. Hematoxylin-eosin ...read more
Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Detection of TEM7 with TEM7 antibody. Human HCT-116 cell lysate probed with TEM7 antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Formalin-fixed, paraffin-embedded human breast vessel stained with TEM7 antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
197C193 (IM193)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TEM7/PLXDC1 Antibody (197C193 (IM193)) Summary

Immunogen
Amino acids 409-425 (LQNNLSPKTKGTPVHLG) of human TEM7 were used to develop this monoclonal antibody.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PLXDC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
Application Notes
Immunocytochemistry/Immunofluorescence: see Meng et al, 2007. Immunohistochemistry (frozen): see Lee et al, 2006. Immunohistochemistry (paraffin): 2-5 ug/ml; see Nanda et al, 2004 and Lee et al, 2005. Immunoprecipitation: see Nanda et al, 2004.
Control
TEM7/PLXDC1 Overexpression Lysate
Publications
Read Publications using
NB100-56557 in the following applications:

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for TEM7/PLXDC1 Antibody (197C193 (IM193))

  • FLJ45632
  • plexin domain containing 1,2410003I07Rik
  • plexin domain-containing protein 1
  • PLXDC1
  • TEM3
  • TEM3FLJ36270
  • TEM7
  • TEM7DKFZp686F0937
  • Tumor endothelial marker 3
  • Tumor endothelial marker 7

Background

Recently, using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al, have identified 46 genes, whose expression is specifically elevated in tumor-associated endothelium. Nine of these genes were prominently expressed only in tumor endothelial cells (EC), but were absent or barely detectable in normal ECs, and named as Tumor Endothelial Markers (TEMs, TEM 1-9). TEM7 (Tumor endothelial marker 7) transcripts are specifically expressed in the endothelium of colorectal cancer, primary cancers of lung, pancreas, breast, and brain. TEM7 is expressed specifically in endothelium of these cancers, whether primary or metastasis. The other six members of this family (TEM1, 3, 4, 5, 8, and 9) also show similar expression pattern in lung and brain tumors, and liver metastasis. Since most of the genes expressed differentially in tumor endothelium are also expressed during angiogenesis, these newly discovered genes might provide important resources for basic and clinical studies of human angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TEM7/PLXDC1 Antibody (NB100-56557)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-Fr
(2)
IHC-P
(2)
WB
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-56557 Applications Species
Carpenter RL, Paw I, Zhu H et al. The gain-of-function GLI1 transcription factor TGLI1 enhances expression of VEGF-C and TEM7 to promote glioblastoma angiogenesis. Oncotarget 2015 Sep 8 [PMID: 26093087] (IHC) IHC
Mehran R, Nilsson M, Khajavi M et al. Tumor endothelial markers define novel subsets of cancer-specific circulating endothelial cells associated with antitumor efficacy. Cancer Res. 2014 Mar 13 [PMID: 24626092] (IHC-Fr, Mouse, Human)

Details:
Mouse zenograft tumor models with human and mouse tumor cells & normal mouse lung, Fig 1.
IHC-Fr Mouse, Human
Yamaji Y, Yoshida S, Ishikawa K et al. TEM7 (PLXDC1) in neovascular endothelial cells of fibrovascular membranes from patients with proliferative diabetic retinopathy. Invest Ophthalmol Vis Sci. 2008 Jul [PMID: 18316703] (IHC-P, Human)

Details:
IHC (paraffin), human fibrovascular membranes, Figs. 3, 4, 5.
IHC-P Human
Fuchs Bruno, Mahlum Eric, Halder Chandralekha et al. High expression of tumor endothelial marker 7 is associated with metastasis and poor survival of patients with osteogenic sarcoma. Gene. 2007 Sep 15 [PMID: 17560052] (Human) Human
Meng F, Henson R, Patel T. Chemotherapeutic stress selectively activates NF-kappa B-dependent AKT and VEGF expression in liver cancer-derived endothelial cells. Am J Physiol Cell Physiol. 2007 Aug [PMID: 17537803] (WB, ICC/IF)

Details:
WB (Fig 1A): AML12, normal mouse liver hepatocytes, and Hepa 1-6 mouse liver hepatoma cell lines, and mouse liver endothelial cells IF/ICC (Fig 1B): mouse liver endothelial cell monolayers.
WB, ICC/IF
Lee HK, Kang DS, Seo IA et al. Expression of tumor endothelial marker 7 mRNA and protein in the dorsal root ganglion neurons of the rat. Neurosci Lett. 2006 Jul 10 [PMID: 16707219] (IHC-Fr) IHC-Fr
Lee HK, Bae HR, Park HK et al. Cloning, characterization and neuronal expression profiles of tumor endothelial marker 7 in the rat brain. Brain Res Mol Brain Res. 2005 May 20 [PMID: 15893603] (IHC-P) IHC-P
Nanda A, Buckhaults P, Seaman S et al. Identification of a binding partner for the endothelial cell surface proteins TEM7 and TEM7R. Cancer Res. 2004 Dec 1 [PMID: 15574754] (WB, Human)

Details:
WB: TEM7 transfected 293 cells (Figs 1C and 3B); human colon cancer and normal colon tissue lysates (Fig 1D), IP: TEM7 tranfected 293 cells (Figs 1C and 3B); IHC-paraffin: normal/tumor colon, esophagus and lung tissues (Fig 2A).
WB Human

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TEM7/PLXDC1 Antibody (NB100-56557) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-33228AF350
Alexa Fluor 405 NB100-56557AF405
Alexa Fluor 488 NB100-56557AF488
Alexa Fluor 532 NBP2-33228AF532
Alexa Fluor 594 NBP2-33228AF594
Alexa Fluor 647 NB100-56557AF647
Alexa Fluor 700 NB100-56557AF700
Alexa Fluor 750 NBP2-33228AF750
Biotin NB100-56557B
DyLight 350 NB100-56557UV
DyLight 405 NB100-56557V
DyLight 488 NB100-56557G
DyLight 550 NB100-56557R
DyLight 594 NB100-56557DL594
DyLight 650 NB100-56557C
DyLight 680 NB100-56557FR
DyLight 755 NB100-56557IR
FITC NB100-56557F
HRP NB100-56557H
Janelia Fluor 549 NBP2-33228JF549
Janelia Fluor 646 NBP2-33228JF646

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Bioinformatics Tool for TEM7/PLXDC1 Antibody (NB100-56557)

Discover related pathways, diseases and genes to TEM7/PLXDC1 Antibody (NB100-56557). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol PLXDC1
Entrez
Uniprot
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