TCF7L2 Antibody [DyLight 488]

• Reactivity: Hu, Mu, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 488

TCF7L2 Antibody [DyLight 488] Summary

• Immunogen: Synthetic peptide made to an internal portion of human TCF7L2 (within residues 150-200). [Swiss-Prot# Q9NQB0]
• Predicted Species: Mouse (93%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TCF7L2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Western Blot
• Application Notes: This TCF7L2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~55 kDa. In ICC/IF cytoplasmic staining was observed.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Immunogen displays the following percentage of sequence identity for non-tested species: mouse (93%) and bovine (93%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TCF7L2 Antibody [DyLight 488]

• HMG box transcription factor 4
• hTCF-4
• T-cell factor 4
• T-cell factor-4 variant A
• T-cell factor-4 variant B
• T-cell factor-4 variant C
• T-cell factor-4 variant D
• T-cell factor-4 variant E
• T-cell factor-4 variant H
• T-cell factor-4 variant I
• T-cell factor-4 variant J
• T-cell factor-4 variant K
• T-cell factor-4 variant L
• T-cell factor-4 variant M
• T-cell factor-4 variant X2
• T-cell-specific transcription factor 4
• TCF-4T-cell factor-4 variant F
• TCF4T-cell factor-4 variant G
• transcription factor 7-like 2 (T-cell specific, HMG-box)
• transcription factor 7-like 2

Background

TCF7L2, or TCF4, is a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. TCF7L2 has been implicated in glucose homeostasis through the regulation of pro-glucagon gene expression, which encodes glucagon-like peptide 1 (GLP-1). Genetic variants of TCF7L2 have been linked to type 2 diabetes, and it has specifically been shown to have an important role in the regulation of both beta-cell survival and function (4). Several splicing isoforms for TCF7L2 have been shown to be differentially expressed in tissues during cancer progression (3), and gene targeting studies indicate that TCF7L2 is required to maintain the crypt stem cells of the small intestine (1, 2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCF7L2