ZnT-8/SLC30A8 Antibody (815039) [Unconjugated]

ZnT-8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human ZnT-8 Monoclonal Antibody (Catalog # MAB7936) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

PANC-1 human cell line was stained with Mouse Anti-Human ZNT-8 Monoclonal Antibody (Catalog # MAB7936, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: IHC, CyTOF-ready, Flow
• Clone: 815039
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ZnT-8/SLC30A8 Antibody (815039) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human ZnT‑8
  Lys268-Pro359
  Accession # Q8IWU4
• Specificity: Detects human ZnT‑8 in ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC30A8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZnT-8/SLC30A8 Antibody (815039) [Unconjugated]

• SLC30A8
• solute carrier family 30 (zinc transporter), member 8
• Solute carrier family 30 member 8
• zinc transporter 8
• zinc transporter ZnT-8
• ZnT8
• ZnT-8
• ZNT8ZnT-8

Background

ZnT-8 (Zinc Transporter 8; also SLC30A8) is a 35-40 kDa member of the SLC (solute carrier)-30A subfamily, CDF family of proteins. It is expressed by pancreatic beta ‑cells and alpha -cells, B cells, and adipocytes and is known to play a role in Zn transport. In particular, ZnT-8 appears to transport zinc from the cytosol into secretory vesicles which, in the case of beta -cells, provides a necessary component for proper insulin processing and granule storage. Furthermore, it appears to facilitate glucose‑mediated insulin release. Human ZnT-8 is a multipass transmembrane (TM) protein that is 369 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-79) followed by six TM segments (aa 80-266) and a 103 aa C-terminal cytoplasmic tail. There is a key His-rich motif in the second cytoplasmic loop (aa 197‑205). ZnT-8 forms homodimers and possibly oligomers. There is one alternative start site at Met50. Over aa 268-359, human ZnT-8 shares 79% aa sequence identity with mouse ZnT-8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC30A8
• Uniprot:
  • Q8IWU4(Human)