TCF-3/E2A Antibody - BSA Free

Western Blot: TCF-3/E2A Antibody [NBP2-38961] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TCF3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-38961] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-38961] - Staining of human testis shows distinct nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-38961] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-38961] - Staining of human testis shows moderate to strong nuclear positivity in spermatogonia.

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-38961] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

• Reactivity: Hu
• Applications: WB, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TCF-3/E2A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TCF-3/E2A Antibody - BSA Free (NBP2-38961) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: LAGSTSLMHNHAALPSQPGTLPDLSRPPDSYSGLGRAGA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TCF3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  TCF-3/E2A Protein (NBP2-38961PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TCF-3/E2A Antibody - BSA Free

• bHLHb21
• bHLHb21TCF-3
• Class B basic helix-loop-helix protein 21
• E2A immunoglobulin enhancer-binding factor E12/E47
• E2A
• E2AKappa-E2-binding factor
• Immunoglobulin enhancer-binding factor E12/E47
• Immunoglobulin transcription factor 1
• ITF1
• ITF1Transcription factor ITF-1
• Kappa-E2-binding factor
• MGC129647
• MGC129648
• Pan2
• TCF-3
• Tcfe2a
• transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Transcription factor 3
• transcription factor E2-alpha
• VDIR
• VDR interacting repressor

Background

TCF3 / E2A encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporatic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCF3
• Uniprot:
  • P15923()