EBF-1 Antibody [Unconjugated]

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and CH‑1 mouse B cell lymphoma cell line, and L1.2 mouse pro‑B cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse EBF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5165) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EBF‑1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

EBF-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse EBF-1
  Arg416-Ser520
  Accession # Q07802
• Specificity: Detects endogenous human and mouse EBF-1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EBF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBF-1 Antibody [Unconjugated]

• COE1
• Collier, Olf and EBF transcription factor 1
• early B-cell factor 1
• Early B-cell factor
• EBF1
• EBF-1
• EBFFLJ41763
• O/E-1
• OE-1
• OLF1
• OLF1FLJ39389
• olfactory neuronal transcription factor 1
• transcription factor COE1

Background

EBF-1 (Early B cell Factor 1; also OLF1 and COE1) is a 65-70 kDa member of the COE family of transcription factors. Although expressed in adipocytes and neurons, it is best studied in B cells where IL-7 acts to promote EBF-1 in pre-proB cells, leading to proB stage development. Mouse EBF-1 is 591 amino acids (aa) in length. It contains one DNA-binding region with an embedded zinc-finger motif (aa 51‑235), a dimerization segment between aa 370‑430, and a Pro/Ser-rich transactivation domain (aa 462‑550). EBF-1 either homodimerizes, or heterodimerizes with EBF-2 and -3. There is an alternate start site at Met134, and an isoform that shows a one aa substitution for aa 252‑259. Over aa 416‑520, mouse EBF-1 shows absolute aa identity to the equivalent sequence in rat and human EBF-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EBF-1 Antibody (AF5165)(2)

Publications using AF5165

• JA Bristol, J Brand, M Ohashi, MR Eichelberg, A Casco, SE Nelson, M Hayes, JC Romero-Mas, DC Baiu, JE Gumperz, EC Johannsen, HQ Dinh, SC Kenney Reduced IRF4 expression promotes lytic phenotype in Type 2 EBV-infected B cells PloS Pathogens, 2022-04-26;18(4):e1010453. 2022-04-26 [PMID: 35472072] (Western Blot, Human)
• Lu F, Chen H, Kossenkov A, DeWispeleare K, Won K, Lieberman P EBNA2 Drives Formation of New Chromosome Binding Sites and Target Genes for B-Cell Master Regulatory Transcription Factors RBP-jkappa and EBF1. PLoS Pathog, 2016-01-11;12(1):e1005339. 2016-01-11 [PMID: 26752713] (Western Blot, Human)

Bioinformatics

• Gene Symbol: EBF1
• Uniprot:
  • Q07802()