Western Blot: ID2 Antibody [NBP2-27194] - Analysis using ID2 antibody. Lysate from human brain in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat brain probed with ID2 antibody at 2 ug/ml. ...read more
Immunocytochemistry/ Immunofluorescence: ID2 Antibody [NBP2-27194] - IF Confocal analysis of A549 cells using ID2 antibody (NBP2-27194, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
The amino acid sequence used as immunogen is 100% homologous in White-cheeked Gibbon, Macaque, Golden Hamster, 89% in New World Monkey, Chicken, and 83% in Opossum.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.2 ml PBS and 0.05% BSA
0.05% Sodium Azide
Protein A purified
Alternate Names for ID2 Antibody
bHLHb26cell growth-inhibiting gene 8
Class B basic helix-loop-helix protein 26
DNA-binding protein inhibitor ID2
DNA-binding protein inhibitor ID-2
helix-loop-helix protein ID2
inhibitor of differentiation 2
Inhibitor of DNA binding 2
inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
ID2 protein belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA bindingThis protein may play a role in negatively regulating cell differentiation. ID2 may be an inhibitor of tissue-specific gene expression. Expression of ID2 is high in early fetal tissues, including those of the central nervous system.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
IF Confocal analysis of A549 cells using ID2 antibody (NBP2-27194, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.