ID2 Antibody

Images

 
Western Blot: ID2 Antibody [NBP2-27194] - Analysis using ID2 antibody. Lysate from human brain in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat brain probed with ID2 antibody at 2 ug/ml. ...read more
Immunocytochemistry/ Immunofluorescence: ID2 Antibody [NBP2-27194] - IF Confocal analysis of A549 cells using ID2 antibody (NBP2-27194, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more

Product Details

Summary
Reactivity Hu, Rt, Bv, Ch, Eq, Ha, Pm, Mu, Po, Pm, Pm, Pm-Or, PmSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ID2 Antibody Summary

Immunogen
A portion of amino acids 60-110 of human ID2 was used as the immunogen for this antibody.
Predicted Species
Mouse (100%), Porcine (94%), Chimpanzee (100%), Orangutan (100%), Monkey (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ID2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2ug/ml
  • Immunocytochemistry/Immunofluorescence
Application Notes
ICC data from customer review.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-27194 in the following applications:

Publications
Read Publication using NBP2-27194.

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in White-cheeked Gibbon, Macaque, Golden Hamster, 89% in New World Monkey, Chicken, and 83% in Opossum.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for ID2 Antibody

  • BHLHB26
  • bHLHb26cell growth-inhibiting gene 8
  • Class B basic helix-loop-helix protein 26
  • DNA-binding protein inhibitor ID2
  • DNA-binding protein inhibitor ID-2
  • GIG8
  • helix-loop-helix protein ID2
  • ID2
  • ID2A
  • ID2H
  • inhibitor of differentiation 2
  • Inhibitor of DNA binding 2
  • inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
  • MGC26389

Background

ID2 protein belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA bindingThis protein may play a role in negatively regulating cell differentiation. ID2 may be an inhibitor of tissue-specific gene expression. Expression of ID2 is high in early fetal tissues, including those of the central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-66897
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-02136
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
MAB7650
Species: Mu
Applications: WB, IHC
H00003400-B01P
Species: Hu, Mu
Applications: WB, ICC/IF
AF6116
Species: Hu
Applications: WB, ICC
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-302
Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NB200-103
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-24695
Species: Hu
Applications: WB, IHC, IHC-P
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NB100-56888
Species: Hu, Mu, Rt, Ca, Eq
Applications: WB
NB100-56511
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO
NBP2-46085
Species: Hu
Applications: WB, IHC
H00027065-M01
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
NBP2-27194
Species: Hu, Rt, Bv, Ch, Eq, Ha, Pm, Mu, Po, Pm, Pm, Pm-Or, Pm
Applications: WB, ICC/IF
Supplier Logo

Publications for ID2 Antibody (NBP2-27194)(1)

Review for ID2 Antibody (NBP2-27194) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-27194:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry ID2 NBP2-27194
Enlarge
5
reviewed by:
Anony- mous
ICC Human 07/01/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of A549 cells using ID2 antibody (NBP2-27194, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ID2 Antibody (NBP2-27194) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional ID2 Products

Bioinformatics Tool for ID2 Antibody (NBP2-27194)

Discover related pathways, diseases and genes to ID2 Antibody (NBP2-27194). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ID2 Antibody (NBP2-27194)

Discover more about diseases related to ID2 Antibody (NBP2-27194).
 

Pathways for ID2 Antibody (NBP2-27194)

View related products by pathway.

PTMs for ID2 Antibody (NBP2-27194)

Learn more about PTMs related to ID2 Antibody (NBP2-27194).
 

Research Areas for ID2 Antibody (NBP2-27194)

Find related products by research area.

Blogs on ID2

There are no specific blogs for ID2, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anony- mous
07/01/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol ID2