TCF-3/E2A Antibody - BSA Free

Western Blot: TCF3/E2A Antibody [NBP2-20585] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/ Immunofluorescence: TCF-3/E2A Antibody [NBP2-20585] - TCF-3/E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TCF3 stained by TCF-3/E2A antibody (NBP2-20585) diluted at 1:600.Blue: Fluoroshield with DAPI .

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NBP2-20585] - TCF-3/E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.TCF3 stained by TCF-3/E2A antibody (NBP2-20585) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: TCF-3/E2A Antibody [NBP2-20585] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF-3/E2A antibody (NBP2-20585) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

TCF3 / E2A antibody immunoprecipitates TCF3 protein in IP experiments.IP samples: Jurkat whole cell extractA. 35 ug Jurkat whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of TCF3 protein by 4 ug TCF3 / E2A antibody (NBP2-20585)7.5 % SDS-PAGEThe immunoprecipitated TCF3 protein was detected by TCF3 / E2A antibody (NBP2-20585) diluted at 1:1000.[EasyBlot anti-rabbit IgG was used as a secondary reagent]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TCF-3/E2A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TCF-3/E2A Antibody - BSA Free (NBP2-20585) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TCF3. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TCF3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (83%)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TCF-3/E2A Antibody - BSA Free

• bHLHb21
• bHLHb21TCF-3
• Class B basic helix-loop-helix protein 21
• E2A immunoglobulin enhancer-binding factor E12/E47
• E2A
• E2AKappa-E2-binding factor
• Immunoglobulin enhancer-binding factor E12/E47
• Immunoglobulin transcription factor 1
• ITF1
• ITF1Transcription factor ITF-1
• Kappa-E2-binding factor
• MGC129647
• MGC129648
• Pan2
• TCF-3
• Tcfe2a
• transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Transcription factor 3
• transcription factor E2-alpha
• VDIR
• VDR interacting repressor

Background

The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TCF3
• Uniprot:
  • P15923()