TBC1D4 [p Thr642] Antibody (SR1272) - BSA Free

Western Blot: TBC1D4 [p Thr 642] Antibody (SR1272) [NBP3-22607] - Western blot analysis using NBP3-22607 in 293T treated with insulin cell lysate.

• Reactivity: Hu
• Applications: WB
• Clone: SR1272
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TBC1D4 [p Thr642] Antibody (SR1272) - BSA Free Summary

• Description: Novus Biologicals Rabbit TBC1D4 [p Thr642] Antibody (SR1272) - BSA Free (NBP3-22607) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-TBC1D4 [Thr642] (Uniprot #: O60343)
• Modification: p Thr642
• Specificity: Detects endogenous levels of total phospho-TBC1D4 [Thr642]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TBC1D4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TBC1D4 [p Thr642] Antibody (SR1272) - BSA Free

• Akt substrate of 160 kDa
• AS160Acrg embryonic lethality minimal region ortholog
• DKFZp779C0666
• KIAA0603TBC (Tre-2, BUB2, CDC16) domain-containing protein
• TBC1 domain family member 4
• TBC1 domain family, member 4

Background

AS160, also known as TBC1D4, is a 160 kDa Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. AS-160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking. Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function. Phosphorylation of Thr642 is also important in mediating TBC1D4 redistribution from low density microsomes to the cytosol in adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TBC1D4