TBC1D4 Antibodies
We offer TBC1D4 Antibodies for use in common research applications: Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each TBC1D4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TBC1D4 Antibodies can be used in a variety of model species: Human. Use the list below to choose the TBC1D4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TBC1D4 polyclonal antibodies and browse our TBC1D4 monoclonal antibody catalog.
Alternate Names for TBC1D4 Antibodies
anti-TBC1D4 antibody, anti-Akt substrate of 160 kDa antibody, anti-AS160Acrg embryonic lethality minimal region ortholog antibody, anti-DKFZp779C0666 antibody, anti-KIAA0603TBC (Tre-2, BUB2, CDC16) domain-containing protein antibody, anti-TBC1 domain family member 4 antibody, anti-TBC1 domain family, member 4 antibody
11 Results for "tbc1d4" in Primary Antibodies
![Western Blot: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Western-Blot-NBP2-00597-img0004.jpg "Western Blot: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.")
![Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody (OTI5E6) [NBP2-00597] - TBC1D4 Antibody (5E6) [NBP2-00597] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TBC1D4.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Immunocytochemistry-Immunofluorescence-NBP2-00597-img0006.jpg)
![Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TBC1D4 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Flow-Cytometry-NBP2-00597-img0003.jpg)
![Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - Analysis of Hela cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Flow-Cytometry-NBP2-00597-img0001.jpg)
![Flow Cytometry: TBC1D4 Antibody (5E6) [NBP2-00597] - Analysis of Jurkat cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/TBC1D4-Antibody-5E6-Flow-Cytometry-NBP2-00597-img0002.jpg)
![Western Blot: TBC1D4 Antibody [NB100-57094] - (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Western-Blot-NB100-57094-img0002.jpg "Western Blot: TBC1D4 Antibody [NB100-57094] - (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: TBC1D4 Antibody [NB100-57094] - (3.8ug/ml) staining of Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Immunohistochemistry-Paraffin-NB100-57094-img0003.jpg)
![Western Blot: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Western blot analysis of TBC1D4 on different lysates with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 starve overnight then treated with 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 147 kDa Observed band size: 160 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32749_rabbit-tbc1d4-p-thr642-mab-je60-97-278202415212425.jpg "Western Blot: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Western blot analysis of TBC1D4 on different lysates with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 starve overnight then treated with 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 147 kDa Observed band size: 160 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TBC1D4 [p Thr642] Antibody (JE60-97) [NBP3-32749] - Immunocytochemistry analysis of NIH/3T3 cells starved overnight then treated with or without 100nM Calyculin A for 50 minutes add 100ng/mL insulin for 20 minutes labeling TBC1D4 with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TBC1D4 antibody (NBP3-32749) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32749_rabbit-tbc1d4-p-thr642-mab-je60-97-29202410484887.jpg)
![Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody [NBP3-35805] - Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35805_rabbit-tbc1d4-pab-912202414213796.jpg "Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody [NBP3-35805] - Immunofluorescence analysis of L929 cells using TBC1D4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: TBC1D4 Antibody [NBP3-35805] - Western blot analysis of lysates from Rat skeletal muscle, using TBC1D4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35805_rabbit-tbc1d4-pab-912202415133469.jpg)
![Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody [NBP2-38460] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38460-img0005.jpg "Immunocytochemistry/Immunofluorescence: TBC1D4 Antibody [NBP2-38460] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.")
![Immunohistochemistry-Paraffin: TBC1D4 Antibody [NBP2-38460] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Immunohistochemistry-Paraffin-NBP2-38460-img0004.jpg)
![Western Blot: TBC1D4 [p Ser588] Antibody [NBP3-05451] - Western blot analysis of extracts of HeLa cells, using TBC1D4 antibody (NBP3-05451) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/TBC1D4-[p-Ser588]-Antibody-Western-Blot-NBP3-05451-img0001.jpg "Western Blot: TBC1D4 [p Ser588] Antibody [NBP3-05451] - Western blot analysis of extracts of HeLa cells, using TBC1D4 antibody (NBP3-05451) at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: TBC1D4 Antibody [NBP2-38880] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TBC1D4-Antibody-Immunohistochemistry-Paraffin-NBP2-38880-img0002.jpg "Immunohistochemistry-Paraffin: TBC1D4 Antibody [NBP2-38880] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry: TBC1D4 Antibody - BSA Free [NBP2-81935] - Immunohistochemistry of TBC1D4 in human prostate tissue with TBC1D4 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81935_rabbit-polyclonal-tbc1d4-antibody-2711202420171775.jpg "Immunohistochemistry: TBC1D4 Antibody - BSA Free [NBP2-81935] - Immunohistochemistry of TBC1D4 in human prostate tissue with TBC1D4 antibody at 10 ug/mL.")
![Western Blot: TBC1D4 [p Thr 642] Antibody (SR1272) [NBP3-22607] - Western blot analysis using NBP3-22607 in 293T treated with insulin cell lysate.](http://images.novusbio.com/fullsize/nbp3-22607_rabbit-tbc1d4-p-thr-642-mab-sr1272-5720231531231.jpg "Western Blot: TBC1D4 [p Thr 642] Antibody (SR1272) [NBP3-22607] - Western blot analysis using NBP3-22607 in 293T treated with insulin cell lysate.")