T-bet/TBX21 Antibody (JE60-20)

Western Blot: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Western blot analysis of T-bet/TBX21 on different lysates with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Jurkat cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32908) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32908) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Immunocytochemistry analysis of NK-92 (positive) and K-562 (negative) labeling T-bet/TBX21 with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-T-bet/TBX21 antibody (NBP3-32908) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: T-bet/TBX21 Antibody (JE60-20) [NBP3-32908] - Flow cytometric analysis of NK-92 cells labeling T-bet/TBX21. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32908, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: JE60-20
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

T-bet/TBX21 Antibody (JE60-20) Summary

• Description: Novus Biologicals Rabbit T-bet/TBX21 Antibody (JE60-20) (NBP3-32908) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human T-bet/TBX21 aa 21-770 / 535. (Uniprot: Q9UL17)
• Localization: Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TBX21
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:1000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for T-bet/TBX21 Antibody (JE60-20)

• Tbet
• T-bet
• TBETT-PET
• TBLYM
• TBLYMT-cell-specific T-box transcription factor T-bet
• T-box 21
• T-box expressed in T cells
• T-box protein 21
• T-box transcription factor TBX21
• TBX21
• T-PET
• Transcription factor TBLYM

Background

Tbx21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for T-bet/TBX21 Antibody (NBP3-32908). (Showing 1 - 1 of 1 FAQ).

1. Do you make a Tbx21 ab for FACS for porcine?
   • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Bioinformatics

• Gene Symbol: TBX21