STAT5A [p Tyr694] Antibody - BSA Free

Western Blot: STAT5A [p Tyr694] Antibody [NB100-82219] - Analysis of extracts from K562 cells untreated or treated with G-CSF using STAT5A(Phospho-Tyr694) Antibody.

Immunohistochemistry-Paraffin: STAT5A [p Tyr694] Antibody [NB100-82219] - STAT5A [p Tyr694] Antibody [NB100-82219] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82219(left) or the same antibody preincubated with blocking peptide(right).

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

STAT5A [p Tyr694] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit STAT5A [p Tyr694] Antibody - BSA Free (NB100-82219) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human STAT5A around the phosphorylation site of tyrosine 694 (D-G-YP-V-K).
• Modification: p Tyr694
• Specificity: Detects endogenous levels of STAT5A only when phosphorylated at tyrosine 694.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STAT5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  STAT5A Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for STAT5A [p Tyr694] Antibody - BSA Free

• MGF
• signal transducer and activator of transcription 5A
• STAT5
• STAT5a

Background

Stat 5a protein is a transcription factor activated by hormone and cytokines. Two highly related, but distinct Stat 5 genes (Stat 5a and Stat 5b) were identified in mouse. The amino acid sequences of Stat 5a and Stat 5b show 96% sequence similarity, and both proteins are co expressed in most tissues of both virgin and lactating mice. However, differential accumulation of Stat 5a and Stat 5b mRNA has been reported for both muscle and mammary tissue. Stat 5a is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAT5A
• Entrez:
  • 6776(Human)
  • 20850(Mouse)
  • 24918(Rat)
• Uniprot:
  • P42229(Human)