STAT3 Antibody (9D8)


Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Human breast cancer cell MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Stat3 at Y705 and total Stat3 were detected by western blot. From more
Immunocytochemistry/ Immunofluorescence: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of STAT3 using anti-STAT3 (9D8) monoclonal antibody (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% more
Immunohistochemistry-Paraffin: STAT3 Antibody (9D8) [NBP2-22471] - Biopsies of normal and cancer tissues.
Chromatin Immunoprecipitation: STAT3 Antibody (9D8) [NBP2-22471] - Analysis performed using cross-linked chromatin from rat hepatoma cells treated with insulin. IP performed using a multiplex microplate Matrix ChIP more
Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of 25ug HepG2 total lysate.
Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of 25ug of various whole cell lysates.
Western Blot: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of 25ug of U2-OS lysate from STAT3 SMART pool siRNA transfected or non-targeting control transfected U2-OS cells onto a 4-20% Tris-HCl polyacrylamide gel.
Immunoprecipitation: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of STAT3 was performed on HepG2 cells. The antigen: antibody complex was formed by incubating 750ug whole cell lysate with 2ug of mouse monoclonal more

Product Details

Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
1 mg/ml

Order Details

STAT3 Antibody (9D8) Summary

Residues 655-770 from human recombinant protein expressed in bacteria
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation 1 - 3 ul
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:1600
  • Immunohistochemistry-Paraffin 1:1600
  • Immunoprecipitation 2 ug
  • Knockdown Validated
  • Western Blot 1:5000
Application Notes
WB from verified customer review.
Read Publications using
NBP2-22471 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS with 1 mg/ml BSA
0.05% Sodium Azide
1 mg/ml
Protein A purified

Alternate Names for STAT3 Antibody (9D8)

  • Acute-phase response factor
  • APRFMGC16063
  • DNA-binding protein APRF
  • FLJ20882
  • HIES
  • signal transducer and activator of transcription 3 (acute-phase responsefactor)
  • signal transducer and activator of transcription 3
  • STAT3


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STAT3 Antibody (NBP2-22471)(3)

Reviews for STAT3 Antibody (NBP2-22471) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for STAT3 Antibody (NBP2-22471). (Showing 1 - 1 of 1 FAQs).

  1. For antibodies : STAT5b H00006777-M03; STAT 5a NBR2-00622; STAT3 NBP2-22471; STAT 6 H00006778-M01 - do these detect phosphorylated , unphosphorylated or both forms of STATs?
    • Antibodies H00006777-M03, NBR2-00622,  NBP2-22471,  and H00006778-M01 were raised to recognize total STAT proteins, they do not differentiate between any posttranslational modifications and will recognize both phosphorylated and non-phosphorylated protein. 

Secondary Antibodies


Isotype Controls

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Blogs on STAT3.

Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

HLA G - mediating immune tolerance during pregnancy
Human leukocyte antigen G (HLA G) is a major histocompatibility complex (MHC) class I molecule that is primarily expressed in the placenta and is essential for the immune tolerance of the fetus during pregnancy. Unlike many HLA genes, HLA G has re...  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

Thymoquinone: A Natural Product with Diverse Therapeutic Potential
Thymoquinone (2-isopropyl-5-methyl-1,4-benzoquinone, or TQ) is derived from the seeds of the black cumin plant Nigella sativa. It has been reported to have a number of beneficial properties including anti-oxidative, anti-inflammatory and anti-tumorige...  Read full blog post.

Survivin Acetylation: Affecting Apoptosis and Cancer
Survivin (BRIC5) is an inhibitor of apoptosis that also promotes cellular adaptation under stressful conditions and helps to regulate cell division. Recently, an antibody study by Dr. H Wang et al. at Brown University [PMID: 20826784] found that Survi...  Read full blog post.

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Gene Symbol STAT3