STAM-1 Antibody (29C678)

Western Blot: STAM-1 Antibody (29C678) [NB100-56533] - Western blot analysis of HeLa lysate (35ug per lane, RIPA buffer) using STAM antibody (NB100-56533) at 1ug/ml. Band observed at ~65kDA. (Expected MW of 59.2kDa according to NP_003464.1).

• Reactivity: Hu
• Applications: WB
• Clone: 29C678
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

STAM-1 Antibody (29C678) Summary

• Description: Novus Biologicals Mouse STAM-1 Antibody (29C678) (NB100-56533) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a synthetic peptide corresponding to amino acids 149-165 of human STAM.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAM
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
• Application Notes: A 59 kDa band is observed.
• Control:

  293 Whole Cell Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for STAM-1 Antibody (29C678)

• DKFZp686J2352
• HSE1 Homolog
• signal transducing adapter molecule 1
• signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
• STAM
• STAM1
• STAM-1
• STAM1STAM-1

Background

STAM is a signal transducing adaptor molecule, that contains an Src homology 3 (SH3) domain and immunoreceptor tyrosine-based activation motif (ITAM). STAM is associated with Jak3 and Jak2 tyrosine kinases via its ITAM region and phosphorylated by Jak3 and Jak2 upon stimulation with IL-2 and GM-CSF, respectively. An SH3 deletion mutant of STAM confers a dominant-negative effect on DNA synthesis mediated by IL-2 and GM-CSF. Furthermore, the wild-type STAM, but not STAM mutants deleted of SH3 and ITAM, significantly enhances c-myc induction mediated by IL-2 and GM-CSF. These results strongly implicate STAM in the signaling pathways for cell growth and c-myc induction immediately downstream of the Jaks associated with the cytokine receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAM
• Uniprot:
  • Q92783(Human)