SREBP1 Antibody


Western Blot: SREBP1 Antibody [NB100-74542] - Analysis of 25 ug of mouse liver (lane 1), rat liver (lane 2) and HepG2 (lane 3) cell lysates onto an SDS polyacrylamide gel.
Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542] - Analysis of SREBP1 (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542] - Analysis of SREBP1 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody more
Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542] - Analysis of SREBP1 (green) showing staining in the cytoplasm of HepG2 cells (right) compared to a negative control without primary antibody (left).

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
1 mg/ml

Order Details

SREBP1 Antibody Summary

Synthetic peptide corresponding to residues M(32) L Q L I N N Q D S D F P G L F(47) of mouse SREBP 1.
Cell Membrane, Endoplasmic reticulum, Golgi Apparatus and Nuclear. Multi-pass membrane protein. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Immunogen affinity purified
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  • Western Blot 1:500 - 1:5000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
Application Notes
WB: Detects an approx. 68 and 120 kDa protein representing SREBP 1 in mouse and rat liver samples as well as rat kidney samples. A predominent band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished.
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NB100-74542 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS containing 1 mg/ml BSA
0.05% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for SREBP1 Antibody

  • BHLHD1
  • Class D basic helix-loop-helix protein 1
  • SREBP 1c
  • SREBP-1
  • sterol regulatory element binding protein-1
  • sterol regulatory element binding transcription factor 1
  • sterol regulatory element-binding protein 1
  • Sterol regulatory element-binding transcription factor 1


Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SREBP1 Antibody (NB100-74542)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Blogs on SREBP1.

SREBP2 - regulating cholesterol homeostasis and lipid metabolism
Sterol regulatory element-binding proteins (SREBP) are important transcription factors regulating the synthesis and uptake of lipids including cholesterol. This essential role in lipid metabolism makes investigations into the functions SREBPs im...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

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Gene Symbol SREBF1