SREBP2 Antibody (1D2)

Images

 
Western Blot: SREBP2 Antibody (1D2) [NBP1-54446] - Lane1: HEp-2 cells. Lane 2: MCF-7 cells. Lane 3: LNCaP cells. Lane 4: HT1080 cells. Lane 5: ZR75-1 cells.
Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody (1D2) [NBP1-54446] - SREBP2 (1D2) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more

Product Details

Summary
Reactivity Hu, ChSpecies Glossary
Applications WB, ELISA, GS, ICC/IF, IP
Clone
1D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

SREBP2 Antibody (1D2) Summary

Immunogen
Human SREBP2 protein (amino acids 48-403). [UniProt# Q12772]
Localization
Endoplasmic reticulum and Golgi apparatus membranes,cytoplasmic vesicle. Mature/processed form localized to nucleus.
Specificity
Recognizes the precursor and mature forms of human SREBP2.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SREBF2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 - 5 ug/mL
  • ELISA
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunoprecipitation 10 ug/mg protein
Application Notes
In WB, this antibody is expected to detect the precursor as well as the mature forms of SREBP2. Use of this antibody in EMSA/GS (Gel Supershift Assay) and IP applications has been reported in PMIDs 15333705 and 15213220, respectively.
Publications
Read Publications using
NBP1-54446 in the following applications:

  • IP
    1 publication
  • WB
    14 publications

Reactivity Notes

Chicken was reported in PMIDs 15333705 and 11907029.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for SREBP2 Antibody (1D2)

  • bHLhd2
  • bHLHd2sterol regulatory element-binding protein 2
  • Class D basic helix-loop-helix protein 2
  • SREBF2
  • SREBP2
  • SREBP2SREBP-2
  • sterol regulatory element binding transcription factor 2
  • Sterol regulatory element-binding transcription factor 2

Background

Sterol regulatory element-binding proteins (SREBPs) compose a family of transcriptional factors that regulate the expression of various genes required for the synthesis of phospholipids, fatty acids, and cholesterol. Out of the three isoforms present (SREBP-1a, SREBP-1c and SREBP-2), SREBP-2 has been implicated more directly in the synthesis of cholesterol during metabolism. Similar to SREBP-1a and SREBP-1c, SREBP-2 acts by first binding to sterol regulatory DNA sequences, and subsequently up-regulating the synthesis of enzymes involved in sterol biosynthesis. SREBP-2 is expressed in both the cytoplasm and nucleus on a subcellular level, depending on the state of the protein. It has been known to interact and form a complex with the SCAP protein at the ER, in turn inhibiting interaction between SCAP and SEC24B. This interaction prevents the cleavage of SREBP-2, which results in the synthesis of cholesterol and uptake of LDL despite high levels of present sterols. In recent research, new functions for the SREBP family have offered insight on the effect of lipid metabolism on various pathophysiological diseases (cancers, steatosis and immunity, PMID: 22154484).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SREBP2 Antibody (NBP1-54446)(14)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(14)
All Applications
Filter By Species
Human
(12)
Chicken
(2)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP1-54446 Applications Species
Du X, Kazim AS, Dawes IW et al. The AAA ATPase VPS4/SKD1 regulates endosomal cholesterol trafficking independently of ESCRT-III.. Traffic. 2013 Jan 1 [PMID: 23009658] (WB, Human) WB Human
Du X, Kazim AS, Brown AJ, Yang H. An essential role of Hrs/Vps27 in endosomal cholesterol trafficking.. Cell Rep. 2012 Jan 26 [PMID: 22832105] (WB, Human)

Details:
SREBP2 antibody (clone 1D2) was used for WB analysis of lysates from HeLa cells that were transfected with siNPC1, siHrs, or a universal control siCtrl, and were then stimulated with 50ug/ml of LDL (Figure 3C).
WB Human
Luu W, Sharpe LJ, Stevenson J, Brown AJ et al. Akt acutely activates the cholesterogenic transcription factor SREBP-2.. Biochim Biophys Acta. 2012 Feb 1 [PMID: 22005015] (WB, Human) WB Human
Song BL, DeBose-Boyd RA. Insig-dependent ubiquitination and degradation of 3-hydroxy-3-methylglutaryl coenzyme a reductase stimulated by delta- and gamma-tocotrienols.. J Biol Chem. 2006 Sep 1 [PMID: 16831864] (WB, Human) WB Human
Song BL, Javitt NB, DeBose-Boyd RA. Insig-mediated degradation of HMG CoA reductase stimulated by lanosterol, an intermediate in the synthesis of cholesterol.. Cell Metab. 2005 Mar 1 [PMID: 16054061] (WB, Human)

Details:
SREBP2 antibody (clone 1D2) was used at 5ug/ml concentration in WB application on nuclear/membrane fractions of SV-589 cells (an immortalized line of human fibroblasts expressing the SV40 large T antigen) that were exposed to medium A containing 10% lipoprotein-deficient serum/LPDS, 50 uM compactin, 10 mM mevalonate, and different different concentrations of 25-hydroxycholesterol, lanosterol, or 24,25-dihydrolanosterol (Figure 2A).
WB Human
Sun LP, Li L, Goldstein JL, Brown MS. Insig required for sterol-mediated inhibition of Scap/SREBP binding to COPII proteins in vitro.. J Biol Chem. 2005 Jul 15 [PMID: 15899885] (WB, Human) WB Human
Adams CM, Reitz J, De Brabander JK et al. Cholesterol and 25-hydroxycholesterol inhibit activation of SREBPs by different mechanisms, both involving SCAP and Insigs.. J Biol Chem. 2004 Dec 10 [PMID: 15452130] (WB, Human) WB Human
Zhang Y, Hillgartner FB. Starvation and feeding a high-carbohydrate, low-fat diet regulate the expression sterol regulatory element-binding protein-1 in chickens.. J Nutr. 2004 Sep 1 [PMID: 15333705] (WB, Chicken)

Details:
SREBP2 antibody (clone 1D2) was used for WB analysis of nuclear and membrane fractions from livers of 10- 12 days old chickens subjected to 24 hours of starvation followed by feeding of high-carbohydrate, low-fat diet for 0, 5, and 24 hours (FIGURE 3). The antibody was also used for EMSA /GS (gel mobility shift assays) under similar experimental conditions on nuclear extracts from chick's liver tissue (FIGURE 5).
WB Chicken
Demoulin JB, Ericsson J, Kallin A et al. Platelet-derived growth factor stimulates membrane lipid synthesis through activation of phosphatidylinositol 3-kinase and sterol regulatory element-binding proteins.. J Biol Chem. 2004 Aug 20 [PMID: 15213220] (WB, IP, Human)

Details:
SREBP2 antibody (clone 1D2) was used for Immunoprecipitation and Western blot applications in experiments involving human AG01518 skin fibroblasts stimulated or not with platelet-derived growth factor/PDGF-BB. For IP application, the antibody was used at 10 ug antibody/ 1mg of total sample protein concentration (FIG. 2C, FIG. 4A). See full text for detailed protocol and data images.
WB, IP Human
Sever N, Yang T, Brown MS et al. Accelerated degradation of HMG CoA reductase mediated by binding of insig-1 to its sterol-sensing domain.. Mol Cell. 2003 Jan 1 [PMID: 12535518] (WB, Human) WB Human
Show All 14 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SREBP2 Antibody (NBP1-54446). (Showing 1 - 1 of 1 FAQs).

  1. The species reactivity of this antibody is only human, right? I have done IHC staining for the tissue of SQ prostate cancer in nude mice using SREBP-2 antibody from other companies. But we can detect high background and non-specific staining. So we try to find a new antibody just against human SREBP-2.
    • As long as the tissue you use is human there should not be any back ground noise. If you are using this Ab on mouse tissue you will have non specific binding as the host of this Ab is mouse.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-54446AF350
Alexa Fluor 405 NBP1-54446AF405
Alexa Fluor 488 NBP1-54446AF488
Alexa Fluor 532 NBP1-54446AF532
Alexa Fluor 594 NBP1-54446AF594
Alexa Fluor 647 NBP1-54446AF647
Alexa Fluor 700 NBP1-54446AF700
Alexa Fluor 750 NBP1-54446AF750
Biotin NBP1-54446B
DyLight 350 NBP1-54446UV
DyLight 405 NBP1-54446V
DyLight 488 NBP1-54446G
DyLight 550 NBP1-54446R
DyLight 594 NBP1-54446DL594
DyLight 650 NBP1-54446C
DyLight 680 NBP1-54446FR
DyLight 755 NBP1-54446IR
HRP NBP1-54446H
Janelia Fluor 549 NBP1-54446JF549
Janelia Fluor 646 NBP1-54446JF646

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Discover related pathways, diseases and genes to SREBP2 Antibody (NBP1-54446). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on SREBP2.

SREBP2 - regulating cholesterol homeostasis and lipid metabolism
Sterol regulatory element-binding proteins (SREBP) are important transcription factors regulating the synthesis and uptake of lipids including cholesterol. This essential role in lipid metabolism makes investigations into the functions SREBPs im...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

SREBPs: Global Regulator of Lipid Metabolism
Sterol regulatory element binding proteins (SREBPs) are indirectly required for cholesterol biosynthesis and for uptake and fatty acid biosynthesis. There are three known SREBP isoforms, SREBP1a, 1c and SREBP2; these have different roles in lipid synt...  Read full blog post.

SREBP2: From Cholesterol Homeostasis to Cancer Invasion
Sterol-regulatory-element-binding protein 2 (SREBP2) is a transcription factor that regulates cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g. HMG-CoA. Along with another transcription factor LXR, SREBP...  Read full blog post.

ABCA1 Expression is Down-Regulated by SREBP microRNA
The ABCA1 (ATP-binding cassette transporter-A1) gene encodes a transmembrane protein, which plays a major role in phospholipid homeostasis by regulating cholesterol efflux from the cell. ABCA1 antibody studies have shown ABCA1 expression is up/down re...  Read full blog post.

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Bioinformatics

Gene Symbol SREBF2
Entrez
Uniprot