SREBP2 Antibody (1D2) Summary
Human SREBP2 protein (amino acids 48-403). [UniProt# Q12772]
Endoplasmic reticulum and Golgi apparatus membranes,cytoplasmic vesicle. Mature/processed form localized to nucleus.
Recognizes the precursor and mature forms of human SREBP2.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 2 - 5 ug/mL
- Gel Super Shift Assays
- Immunocytochemistry/Immunofluorescence 10 ug/mL
- Immunoprecipitation 10 ug/mg protein
In WB, this antibody is expected to detect the precursor as well as the mature forms of SREBP2. Use of this antibody in EMSA/GS (Gel Supershift Assay) and IP applications has been reported in PMIDs 15333705 and 15213220, respectively.
|Read Publications using
NBP1-54446 in the following applications:
Chicken was reported in PMIDs 15333705 and 11907029.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for SREBP2 Antibody (1D2)
- bHLHd2sterol regulatory element-binding protein 2
- Class D basic helix-loop-helix protein 2
- sterol regulatory element binding transcription factor 2
- Sterol regulatory element-binding transcription factor 2
Sterol regulatory element-binding proteins (SREBPs) compose a family of transcriptional factors that regulate the expression of various genes required for the synthesis of phospholipids, fatty acids, and cholesterol. Out of the three isoforms present (SREBP-1a, SREBP-1c and SREBP-2), SREBP-2 has been implicated more directly in the synthesis of cholesterol during metabolism. Similar to SREBP-1a and SREBP-1c, SREBP-2 acts by first binding to sterol regulatory DNA sequences, and subsequently up-regulating the synthesis of enzymes involved in sterol biosynthesis. SREBP-2 is expressed in both the cytoplasm and nucleus on a subcellular level, depending on the state of the protein. It has been known to interact and form a complex with the SCAP protein at the ER, in turn inhibiting interaction between SCAP and SEC24B. This interaction prevents the cleavage of SREBP-2, which results in the synthesis of cholesterol and uptake of LDL despite high levels of present sterols. In recent research, new functions for the SREBP family have offered insight on the effect of lipid metabolism on various pathophysiological diseases (cancers, steatosis and immunity, PMID: 22154484).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
View specific protocols for SREBP2 Antibody (NBP1-54446):
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for SREBP2 Antibody (NBP1-54446). (Showing 1 - 1 of 1 FAQs).
The species reactivity of this antibody is only human, right? I have done IHC staining for the tissue of SQ prostate cancer in nude mice using SREBP-2 antibody from other companies. But we can detect high background and non-specific staining. So we try to find a new antibody just against human SREBP-2.
- As long as the tissue you use is human there should not be any back ground noise. If you are using this Ab on mouse tissue you will have non specific binding as the host of this Ab is mouse.
Other Available Formats
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