SR-BI Antibody [Biotin]

Product Discontinued

SR-BI Antibody [Biotin] Summary

• Immunogen: A synthetic peptide made to the C-terminal region of mouse SR-BI (between residues 400-509). [UniProt# Q61009]
• Localization: Cell Membrane; multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SCARB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This SR-BI antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is observed at ~ 82 kDa. Flow Cytometry was reported in scientific literature.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Customers have also reported positive detection in cytosolic fractions from 13-lined ground squirrel liver samples, with an unidentified non-specific band at ~60 kDa.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SR-BI Antibody [Biotin]

• CD36 and LIMPII analogous 1
• CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
• CD36 antigen
• CD36L1
• CD36L1scavenger receptor class B type 1
• CLA1 CD36 antigen-like 1
• CLA-1 HDLQTL6
• CLA1
• Collagen type I receptor, thrombospondin receptor-like 1
• HDLQTL6
• MGC138242
• SCARB1
• scavenger receptor class B type III
• scavenger receptor class B, member 1
• SRB1 scavenger receptor class B member 1
• SR-B1
• SRBI
• SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to the CD36 family and acts as a cell surface lipoprotein receptor for a variety of ligands including high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS). SR-BI mediates selective uptake of HDL cholesteryl (HDL-C) ester in the liver, steroidogenic tissues, and adipose tissue and facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins. SR-BI plays a role in antigen capture and cross-presentation from bacteria, parasites, and viruses such as Hepatitis C. In endothelial cells and macrophages, SR-BI is involved in anti-inflammatory and pro-survival signaling, providing protection against atherosclerosis and clearing apoptotic cells (efferocytosis) (1, 2).

At least 5 different human splice variants have been identified with theoretical molecular weights ranging from 60.9 kDa for the canonical sequence to 45 kDa for an N-terminally truncated variant (SR-BII) (3). The extracellular domain of human SR-BI (CLA-1) shares 80%, 80%, 89%, 86% and 84% aa sequence identity with mouse, rat, porcine, rabbit, and bovine SR-BI, respectively. SR-BI is highly glycosylated and removal of N-linked glycosylation reduces lipid transport.

References

1. Linton MF, Tao H, Linton EF, Yancey PG. (2017) SR-BI: A Multifunctional Receptor in Cholesterol Homeostasis and Atherosclerosis. Trends Endocrinol Metab. 28(6):461-472. PMID: 28259375

2. Hoekstra M, Sorci-Thomas M. (2017) Rediscovering scavenger receptor type BI: surprising new roles for the HDL receptor. Curr Opin Lipidol. 28(3):255-260. PMID: 28301373

3. Shen WJ, Asthana S, Kraemer FB, Azhar S. (2018) Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCARB1