SR-BI Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-113] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Western Blot: SR-BI Antibody [NB400-113] - Detection of SR-BI (80kDa) in mouse testis lysate total protein using NB400-113.
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-113] - Analysis of a FFPE section of human adrenal gland tissue using 1:100 dilution of SR-BI antibody. The staining was developed using HRP-DAB based detection ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-113] - Analysis of a FFPE section of human adrenal gland tissue using 1:100 dilution of SR-BI antibody. The staining was developed using HRP-DAB based detection ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SR-BI Antibody Summary

Immunogen
Adenovirus encoding mouse SR-BI. [UniProt# Q61009]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARB1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:100
  • Block/Neutralize
Application Notes
This SR-BI antibody is useful for Flow Cytometry (PMID: 22622498), Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot and for blocking the binding of ligands to SR-BI. Immunohistochemistry-Frozen was reported in scientific literature.
Reviewed Applications
Read 1 Review rated 4
using
NB400-113 in the following applications:

Publications
Read Publications using
NB400-113 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for SR-BI Antibody

  • CD36 and LIMPII analogous 1
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
  • CD36 antigen
  • CD36L1
  • CD36L1scavenger receptor class B type 1
  • CLA1 CD36 antigen-like 1
  • CLA-1 HDLQTL6
  • CLA1
  • Collagen type I receptor, thrombospondin receptor-like 1
  • HDLQTL6
  • MGC138242
  • SCARB1
  • scavenger receptor class B type III
  • scavenger receptor class B, member 1
  • SRB1 scavenger receptor class B member 1
  • SR-B1
  • SRBI
  • SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to CD36 family and act as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and HDL located in caveolae (regions over plasma membrane). It plays important role in meditating the uptake of HDL-derived cholesterol as well as cholesteryl ester in liver and steroidogenic tissues. It facilitates the flux of free and esterified cholesterol between cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. SR-BI is expressed in endothelial cells, macrophages and dendritic cells also. It has been suggested to be involved in the phagocytosis of apoptotic cells (via its phosphatidylserine binding activity); uptake of lipid soluble vitamins (vitamin E and carotenoids) and pathogen recognition, anti-inflammatory responses and its expression can be modulated by LPS as well as ROS. SR-BI has also been involved in the capture and cross-presentation of antigens from viruses, bacteria and parasites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB400-113
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N

Publications for SR-BI Antibody (NB400-113)(29)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: B/N, Block/Neutralize, FLOW, Flow, ICC/IF, IHC, IHC-Fr, WB.


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B/N
(5)
Block/Neutralize
(1)
FLOW
(4)
Flow
(1)
ICC/IF
(3)
IHC
(1)
IHC-Fr
(1)
WB
(4)
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Human
(9)
Mouse
(10)
Rat
(1)
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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB400-113 Applications Species
Kumaran MS, Bishnoi A, Srivastava N et al. High-density lipoproteins induce miR-223-3p biogenesis and export from myeloid cells: Role of scavenger receptor BI-mediated lipid transfer Atherosclerosis May 2 2019 [PMID: 31096070] (Block/Neutralize, Human) Block/Neutralize Human
Cabou C, Honorato P, Briceno L et al. Pharmacological inhibition of the F1 -ATPase/P2Y1 pathway suppresses the effect of apolipoprotein A1 on endothelial nitric oxide synthesis and vasorelaxation Acta Physiol (Oxf) Mar 1 2019 [PMID: 30821416] (B/N, Mouse) B/N Mouse
Morel E, Ghezzal S, Lucchi G et al. Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells. Biochim. Biophys. Acta 2018 [PMID: 29196159] (Flow) Flow
Kuai R, Subramanian C, White PT et al. Synthetic high-density lipoprotein nanodisks for targeted withalongolide delivery to adrenocortical carcinoma Int J Nanomedicine 2017 Sep 18 [PMID: 28919755] (IHC, Human) IHC Human
Robert J, Button EB, Stukas S et al. High-density lipoproteins suppress Abeta-induced PBMC adhesion to human endothelial cells in bioengineered vessels and in monoculture Mol Neurodegener 2017 Aug 22 [PMID: 28830501] (B/N, Human) B/N Human
Tang J, Kuai R, Yuan W et al. Effect of size and pegylation of liposomes and peptide-based synthetic lipoproteins on tumor targeting Nanomedicine 2017 Aug 01 [PMID: 28434931] (ICC/IF, Human) ICC/IF Human
Kadam PD, Chuan HH. Erratum to: Rectocutaneous fistula with transmigration of the suture: a rare delayed complication of vault fixation with the sacrospinous ligament. Int Urogynecol J 2016 Mar [PMID: 26811110]
Cameron SJ, Morrell CN, Bao C et al. A Novel Anti-Inflammatory Effect for High Density Lipoprotein. PLoS ONE. 2015 Dec 18 [PMID: 26680360] (B/N, Human) B/N Human
Pardee AD, Yano H, Weinstein AM et al. Route of antigen delivery impacts the immunostimulatory activity of dendritic cell-based vaccines for hepatocellular carcinoma J Immunother Cancer. 2015 Jul 22 [PMID: 26199728] (FLOW, Human) FLOW Human
Sharma M, Von Zychlinski-Kleffmann A, Porteous CM et al. Lipoprotein(a) upregulates ABCA1 in liver cells via scavenger receptor-B1 through its oxidised phospholipids J. Lipid Res. 2015 Apr 06 [PMID: 25852127] (B/N, Human)

Details:
SR-BI antibody was used for blocking the SR-B1 receptor on HepG2 cells. The cells were preincubated with 5 ug/ml of anti-SR-B1 antibody for 3 hours at 37C prior to lipoprotein(a) treatment. The proteins from the treated cells were then analysed for the ABCA1, PPAR-gamma and LXR alpha expression.
B/N Human
Show All 29 Publications.

Review for SR-BI Antibody (NB400-113) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB400-113:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 08/07/2012
View

Summary

ApplicationWestern Blot
Sample Testedpurified SR-BI
SpeciesMouse
LotF-1

Blocking

Blocking Details5% low fat milk in PBS/Tween-20 (0.1%)

Primary Anitbody

Dilution RatioSR-BI Antibody (NB400-113) 1:1000

Secondary Antibody

Secondary DescriptionMouse anti-rabbit IgG, HRP
Secondary Manufacturer Cat#GE Healthcare NA934V
Secondary Concentration1:10000 dilution

Details

Detection NotesChemiluminescense detection (ECL plus). 30 sec exposure. Positive control - KKB-1 antibody, neg - CD-36 protein

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SR-BI Antibody (NB400-113). (Showing 1 - 5 of 5 FAQs).

  1. Do you have information on epitope of NB400-113?
    • NB400-113 is not epitope mapped and was generating by using an immunogen of full-length mouse SR-BI expressed by adenovirus
  2. Is this Ab identical to the former Ab3811 - Ab 3811 was used by Liisanantti et al. (Alcoholism: Clinical and Experimental Research 2009) is the Ab3811 still available?
    • Yes, Ab3811 from Abcam and the antibody NB400-113 from Novus are one and the same. The Abcam catalog number is our antibody that they are distributing. NB400-113 should perform as indicated in the literature under Ab3811.
  3. Could you please give me information about the molecular weight of SR-BI-antibody (NB400-113)?
    • Encoded by gene SCARB1, SR-BI is a plasma membrane receptor for high density lipoprotein cholesterol (HDL) and its expected molecular weight is 61kD. However, this protein undergoes post-translational modifications including N-glycosylation which causes a significant increase in the molecular mass from 61kD to up to approximately 82kD.
  4. We are interested in using this antibody to perform staining on cells and to detect cells with FACS (flow cytometry). It is indicated on the datasheet that it is possible to use this antibody for flow cytometry, however little information is provided. Could you please inform us on the isotype of the antibody and the concentration we need to use for our FACS experiments?
    • This antibody is a rabbit polyclonal, so the isotype is IgG. We have never tested in-house for flow cytometry. This antibody has been validated for use in Flow Cytometry by other researchers in publications. All the information we have regarding its use in flow cytometry comes from the publications on the datasheet (PMID: 20041149 and 22622498). We recommend 1:50-1:200 for ICC, so this is probably a good range to start your testing with.
  5. Please provide an estimated concentration for NB400-113 Lot G7?
    • As mentioned on the datasheet, this product is an unpurified formulation (whole antiserum) and the exact concentration of antibody is not quantifiable.However, the concentration of antibody in antisera can vary from 1-10 mg/ml.

Secondary Antibodies

 

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Blogs on SR-BI.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

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Anonymous
08/07/2012
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SCARB1
Entrez