SR-BI Antibody Pack

Product Discontinued

SR-BI Antibody Pack Summary

• Description: This pack contains 1 vial each of: NB400-101 (0.1ml), NB400-131 (0.1ml) and NB400-104 (0.1ml).
• Gene: SCARB1

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: See Individual datasheets

Reactivity Notes

See individual datasheets.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Alternate Names for SR-BI Antibody Pack

• CD36 and LIMPII analogous 1
• CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
• CD36 antigen
• CD36L1
• CD36L1scavenger receptor class B type 1
• CLA1 CD36 antigen-like 1
• CLA-1 HDLQTL6
• CLA1
• Collagen type I receptor, thrombospondin receptor-like 1
• HDLQTL6
• MGC138242
• SCARB1
• scavenger receptor class B type III
• scavenger receptor class B, member 1
• SRB1 scavenger receptor class B member 1
• SR-B1
• SRBI
• SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to the CD36 family and acts as a cell surface lipoprotein receptor for a variety of ligands including high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS). SR-BI mediates selective uptake of HDL cholesteryl (HDL-C) ester in the liver, steroidogenic tissues, and adipose tissue and facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins. SR-BI plays a role in antigen capture and cross-presentation from bacteria, parasites, and viruses such as Hepatitis C. In endothelial cells and macrophages, SR-BI is involved in anti-inflammatory and pro-survival signaling, providing protection against atherosclerosis and clearing apoptotic cells (efferocytosis) (1, 2).

At least 5 different human splice variants have been identified with theoretical molecular weights ranging from 60.9 kDa for the canonical sequence to 45 kDa for an N-terminally truncated variant (SR-BII) (3). The extracellular domain of human SR-BI (CLA-1) shares 80%, 80%, 89%, 86% and 84% aa sequence identity with mouse, rat, porcine, rabbit, and bovine SR-BI, respectively. SR-BI is highly glycosylated and removal of N-linked glycosylation reduces lipid transport.

References

1. Linton MF, Tao H, Linton EF, Yancey PG. (2017) SR-BI: A Multifunctional Receptor in Cholesterol Homeostasis and Atherosclerosis. Trends Endocrinol Metab. 28(6):461-472. PMID: 28259375

2. Hoekstra M, Sorci-Thomas M. (2017) Rediscovering scavenger receptor type BI: surprising new roles for the HDL receptor. Curr Opin Lipidol. 28(3):255-260. PMID: 28301373

3. Shen WJ, Asthana S, Kraemer FB, Azhar S. (2018) Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Publications for SR-BI Antibody Pack (NB100-908)(26)

Publications using NB100-908

• Yvan-Charvet, L et al. SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL. J Lipid Res;49(1):107-14. 2008-01-01 [PMID: 17960026] (WB, Mouse)
• Zhang, X et al. Regulation of alternative splicing of liver scavenger receptor class B gene by estrogen and the involved regulatory splicing factors. Endocrinology;148(11):5295-304. 2007-11-01 [PMID: 17673517]
• During A, Harrison EH. Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells. J Lipid Res;48(10):2283-94. 2007-10-01 [PMID: 17644776]
• Atshaves BP, McIntosh AL, Payne HR, Gallegos AM, Landrock K, Maeda N, Kier AB, Schroeder F. SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes. J Lipid Res;48(10):2193-211. 2007-10-01 [PMID: 17609524]
• Nassir F, Wilson B, Han X et al. CD36 is important for fatty acid and cholesterol uptake by the proximal but not distal intestine. J Biol Chem;282(27):19493-501. 2007-07-06 [PMID: 17507371]
• Wang, T et al. Dissection of the pathway required for generation of vitamin A and for Drosophila phototransduction. J Cell Biol 177(2):305-16. 2007-04-23 [PMID: 17452532]
• Parathath, S et al. Effects of amino acid substitutions at glycine 420 on SR-BI cholesterol transport function. J Lipid Res 48(6):1386-95. 2007-06-01 [PMID: 17372332]
• Graham, M et al. Antisense inhibition of proprotein convertase subtilisin/kexin type 9 reduces serum LDL in hyperlipidemic mice. J Lipid Res 48(4):763-7. 2007-04-01 [PMID: 17242417]
• Kipadia, SB et al. Initiation of hepatitis C virus infection is dependent on cholesterol and cooperativity between CD81 and scavenger receptor B type I. J Virol 81(1):374-83. 2007-01-01 [PMID: 17050612]
• Zhong, J et al. Persistent hepatitis C virus infection in vitro: coevolution of virus and host. J Virol;80(22):11082-93. 2006-11-01 [PMID: 16956932]
• Liao, D et al. Hyperhomocysteinemia decreases circulating high-density lipoprotein by inhibiting apolipoprotein A-I Protein synthesis and enhancing HDL cholesterol clearance. Circ Res 99(6):598-606. 2006-09-15 [PMID: 16931800]
• Azhar, S et al. LDL and cAMP cooperate to regulate the functional expression of the LRP in rat ovarian granulosa cells. J Lipid Res;47(11):2538-50. 2006-11-01 [PMID: 16929031]
• Joyce CW 2006. J. Biol. Chem. 281:33053. [PMID: 16928680]
• Sane, AT, at al. Localization role of NPC1L1 in cholesterol absorption in human intestine. Journal of Lipid Research 47: 2112-2120. 2006-01-01 [PMID: 16829661]
• Zhang, C et al. NO-1886 suppressing atherosclerosis in high-fat-high-sucrose/high-cholesterol fed Bama minipigs is related to upregulating ATP-binding cassette transporter A1. J Lipid Res. 2006-01-01 [PMID: 16807312]
• Frank PG et al. Caveolin-1 and regulation of cellular cholesterol homeostasis. Am. J. Physiol. Heart Circ. Physiol. 291(2): H677-686. [PMID: 16603689]
• Li, XA et al. Scavenger receptor BI prevents nitric oxide-induced cytotoxicity and endotoxin-induced death. Circ Res 98(7):e60-5. 2006-04-14 [PMID: 16574909]
• Dichek, HL et al. Attenuated corticosterone response to chronic ACTH stimulation in hepatic lipase-deficient mice: evidence for a role for hepatic lipase in adrenal physiology. Am J Physiol Endocrinol Metab 290(5):E908-15. 2006-05-01 [PMID: 16368783]
• Martin, GG et al. Liver fatty acid binding protein gene ablation potentiates hepatic cholesterol accumulation in cholesterol-fed female mice. Am J Physiol Gastrointest Liver Physiol 290(1):G36-48. 2006-01-01 [PMID: 16123197]
• Wu, MK, Cohen, DE. Phosphatidylcholine transfer protein regulates size and hepatic uptake of high-density lipoproteins. Am J Physiol Gastrointest Liver Physiol 289(6):G1067-74. 2005-12-01 [PMID: 16099870]
• Johansson, L et al. Selective thyroid receptor modulation by GC-1 reduces serum lipids stimulates steps of reverse cholesterol transport in euthyroid mice. PNAS 102(29): 10297-10302. 2005-01-01 [PMID: 16006512]
• Liang, K et al. HMG-CoA reductase inhibition reverses LCAT and LDL receptor deficiencies and improves HDL in rats with chronic renal failure. Am J Physiol Renal Physiol;288(3):F539-44. 2005-03-01 [PMID: 15507547]
• Jordan, BK et al. Wnt4 overexpression disrupts normal testicular vasculature and inhibits testosterone synthesis by repressing steroidogenic factor 1/beta-catenin synergy. Proc Natl Acad Sci U S A 100(19):10866-71. 2003-09-16 [PMID: 12949260]
• Ma, K et al. Endothelial lipase is a major genetic determinant for high-density lipoprotein concentration, structure, and metabolism. Proc Natl Acad Sci U S A 100(5):2748-53. 2003-03-04 [PMID: 12601178]
• Willner, E et al. Deficiency of acyl CoA:cholesterol acyltransferase 2 prevents atherosclerosis in apolipoprotein E-deficient mice. Proc Natl Acad Sci U S A;100(3):1262-7. 2003-02-04 [PMID: 12538880]
• Arai, T et al. Decreased selective uptake of high density lipoprotein cholesteryl esters in apolipoprotein E knock-out mice. Proc Natl Acad Sci U S A;96(21):12050-5. 1999-10-12 [PMID: 10518574]

Bioinformatics

• Gene Symbol: SCARB1