SR-AI/MSR Antibody - BSA Free

Western Blot: SR-AI/MSR Antibody [NB400-159] - Detection of MSR in human liver using NB400-159.

Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody [NB400-159] - Antibody was tested in Raw cells at a 1:50 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.97 mg/ml

SR-AI/MSR Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of human Macrophage Scavenger Receptor I (between residues 300-350). [UniProt P21757]
• Localization: Type II membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MSR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 2 ug/mL
• Application Notes: In Western blot a band is observed at ~50 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Liver Whole Tissue Lysate (Adult Whole Normal)
  SR-AI/MSR Overexpression Lysate

• Control Peptide:

  SR-AI/MSR Peptide (NB400-159PEP)

Reactivity Notes

There have been mixed results (positive and negative) with mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 0.97 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SR-AI/MSR Antibody - BSA Free

• CD204 antigen
• CD204
• Macrophage acetylated LDL receptor I and II
• macrophage scavenger receptor 1
• macrophage scavenger receptor type III
• MSR1
• phSR1
• phSR2
• SCARA1
• SCARA1macrophage scavenger receptor types I and II
• Scavenger receptor class A member 1
• scavenger receptor class A, member 1
• SR-A
• SRAI
• SR-AI

Background

Macrophage Scavenger Receptor I (MSR1, also called CD204 or scavenger receptor A) belongs to scavenger receptor A family and represents phagocytic pattern-recognition receptors (PRRs) expressed predominantly on macrophages and dendritic cells. MSR1 is a single-pass type II membrane glycoprotein capable of binding to a wide range of polyanionic ligands (e.g., modified low density lipoproteins, pathogen-derived molecules), and is implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis, innate immune response as well as Alzheimer's disease. MSR1 mediates the endocytosis of a diverse group of macromolecules including modified LDLs, and scavenger receptors MSR1, CD36 and LOX-1 are three main receptors for oxidized LDL on macrophage membrane wherein MSR1/CD36 account for more than 9/10 of the lipid accumulation in macrophages exposed to oxidized LDL. Defects in MSR1 have also been linked to prostate cancer and Barrett esophagus (BE).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for SR-AI/MSR Antibody (NB400-159). (Showing 1 - 2 of 2 FAQ).

1. What type of mouse was tested with this antibody? Have C57BL/6 mice been tested? Is the immunogen to the helical domain?
   • Some customers have reported success with this antibody in mouse samples, and others have reported that it doesn't work. We have not tested it with mouse in-house, and I don't know whether C57BL/6 mice were used. We approved monkey based on customer feedback, but have not tested in ourselves. The immunogen is from the collagen-like domain.
2. For product# NB400-159, MSRAI antibody, it is reactive with human and primates. The western figure shows that human liver lysate was used. However, when I look at the positive controls, it mentions 2: mouse liver whole tissue lysate and MSR1 overexpression lysate. Is the human liver lysate available?
   • We do have some normal human liver lysates for you to consider. Please see catalog numbers NB820-59232,NB820-59234 and NB820-59233.

Bioinformatics

• Gene Symbol: MSR1