SQLE Antibody

Immunocytochemistry/Immunofluorescence: SQLE Antibody [NBP2-93808] - Analysis of NIH-3T3 cells using SQLE . Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: SQLE Antibody [NBP2-93808] - Analysis of HeLa cells using SQLE . Blue: DAPI for nuclear staining.

Western Blot: SQLE Antibody [NBP2-93808] - Analysis of HepG2, using SQLE antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunocytochemistry/Immunofluorescence: SQLE Antibody [NBP2-93808] -Analysis of HepG2 cells using SQLE Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: SQLE Antibody [NBP2-93808] -Analysis of PC-12 cells using SQLE Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SQLE Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 143-400 of human SQLE (NP_003120.2). SRDGRKVTVIERDLKEPDRIVGEFLQPGGYHVLKDLGLGDTVEGLDAQVVNGYMIHDQESKSEVQIPYPLSENNQVQSGRAFHHGRFIMSLRKAAMAEPNAKFIEGVVLQLLEEDDVVMGVQYKDKETGDIKELHAPLTVVADGLFSKFRKSLVSNKVSVSSHFVGFLMKNAPQFKANHAELILANPSPVLIYQISSSETRVLVDIRGEMPRNLREYMVEKIYPQIPDHLKEPFLEATDNSHLRSMPASFLPPSSVKK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SQLE
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for SQLE Antibody

• EC 1.14.99.7
• ERG1
• FLJ30795
• squalene epoxidaseSE
• squalene monooxygenase

Background

Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SQLE