Splicing Factor 1 Antibody - BSA Free

Western Blot: Splicing Factor 1 Antibody [NBP1-32583] - Sample(30 ug whole cell lysate)A:Raji 7. 5% SDS PAGE, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Splicing Factor 1 Antibody [NBP1-32583] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: splicing factor 1 protein stained by splicing factor 1 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.

• Reactivity: Hu, Mu, Rt, Bv, Rb, Xp, Ze
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Splicing Factor 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Splicing Factor 1 Antibody - BSA Free (NBP1-32583) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Splicing Factor 1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (99%), Rat (99%), Xenopus (96%), Zebrafish (94%), Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SF1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Splicing Factor 1 Antibody - BSA Free

• Mammalian branch point-binding protein
• mBBP
• splicing factor 1
• Transcription factor ZFM1
• ZFM1BBP
• Zinc finger gene in MEN1 locus
• Zinc finger protein 162D11S636
• ZNF162MBBP

Background

Steroidogenic factor-1 (SF-1) regulates multiple genes involved in the adrenal and gonadal development and in the biosynthesis of a variety of hormones, including adrenal and gonadal steroids, anti-Mullerian hormone (AMH), and gonadotropins. SF-1 belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors. In the adult ovary, SF-1 localizes to theca/interstitial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SF1