SPINK5 Antibody - BSA Free

Staining of human cell line HeLa shows localization to vesicles.

Analysis in human tonsil and kidney tissues using Anti-SPINK5 antibody. Corresponding SPINK5 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: SPINK5 Antibody [NBP1-90509] - Staining of human tonsil shows high expression.

Immunohistochemistry-Paraffin: SPINK5 Antibody [NBP1-90509] - Staining of human kidney shows low expression as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SPINK5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SPINK5 Antibody - BSA Free (NBP1-90509) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-SPINK5 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QQEERARAKAKREAAKEICSEFRDQVRNGTLICTREHNPVRGPDGKMHGNKCAMCASVFKLEEEEKKNDKEEKGKVEAEKVKREAVQELCSEYRHYVRNGRLPC
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPINK5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SPINK5 Protein (NBP1-90509PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SPINK5 Antibody - BSA Free

• Kazal type 5
• LEKTI
• LETKI
• NETS
• serine peptidase inhibitor, Kazal type 5
• SPINK5
• VAKTI

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPINK5 Antibody (NBP1-90509)(2)

Publications using NBP1-90509

• Sugihara S, Sugimoto S, Tachibana K et al. TNF-alpha and IL-17A induce the expression of lympho-epithelial Kazal-type inhibitor in epidermal keratinocytes Journal of Dermatological Science 2019-08-01 [PMID: 31501002] (IF/IHC, Human)
• McGovern JA, Meinert C, de Veer SJ et al. Attenuated kallikrein-related peptidase activity disrupts desquamation and leads to stratum corneum thickening in human skin equivalent models. Br J Dermatol 2016-07-21 [PMID: 27442805]

Bioinformatics

• Gene Symbol: SPINK5