SHP-2/PTPN11 [p Tyr542] Antibody [Unconjugated]

Images

 
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 ng/mL Human PDGF-BB (Catalog # 220-BB) for 20 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit ...read more
Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 ng/mL Recombinant Human PDGF‑BB (Catalog # 220-BB) for 20 minutes, loaded at 0.2 mg/mL. A ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

SHP-2/PTPN11 [p Tyr542] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human SHP-2 Y542 site
Modification
p Tyr542
Specificity
Detects human and mouse SHP-2 when phosphorylated at Y542.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTPN11
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publication using
AF3790 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SHP-2/PTPN11 [p Tyr542] Antibody [Unconjugated]

  • BPTP3
  • EC 3.1.3.48
  • MGC14433
  • protein tyrosine phosphatase, non-receptor type 11
  • protein tyrosine phosphatase-2
  • Protein-tyrosine phosphatase 1D
  • Protein-tyrosine phosphatase 2C
  • PTP1D
  • PTP-1D
  • PTP2C
  • PTP-2C
  • PTP2CNS1
  • PTPN11
  • SHP2
  • SHP-2
  • SHP2CFC
  • SHPTP2
  • SH-PTP2
  • SH-PTP2Noonan syndrome 1
  • SH-PTP3
  • tyrosine-protein phosphatase non-receptor type 11

Background

Src-Homology domain-2 containing protein tyrosine Phosphatase 2 (SHP-2), also called protein tyrosine phosphatase, non-receptor type 11 (PTPN11), PTP1D, PTP2C, and SYP, is an enzyme that dephosphorylates tyrosine residues in proteins. The protein contains two Src homology 2 (SH2) domains, which both regulate the activity of the enzyme (1) and allow it to selectively bind to SH2 sites on proteins such as Dok1, IRS1, and the insulin receptor (2). SHP-2 plays a unique stimulatory role in cell signaling. Cells lacking SHP-2 have poor mobility because the hyper-phosphorylation of FAK and other proteins in the focal adhesion complex (3) prevents turnover of cellular attachment points. Without SHP-2, sustained ERK stimulation does not take place (4). The Y992 phosphorylation site of EGFR is a particularly good substrate for SHP-2 (5) and a phosphopeptide containing this sequence can be used to measure the activity of the enzyme (R&D Systems, Catalog # ES006) by detecting release of phosphate (R&D Systems, Catalog # DY996).

  1. Zhao, Z. et al. (1994) J. Biol. Chem. 269:8780.
  2. Clemmons, D.R. and Maile, L.A. (2005) Mol. Endocrinol. 19:1.
  3. von Wichert, G. et al. (2003) EMBO J. 22:5023.
  4. Maroun, C.R. et al. (2000) Mol. Cell. Biol. 20:8513.
  5. Sugimoto, S. et al. (1993) J. Biol. Chem. 269:22771.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SHP-2/PTPN11 Antibody (AF3790)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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(1)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PTPN11