Spi-B Antibody (4G5)

Western Blot: Spi-B Antibody (4G5) [NBP2-37458] - Analysis using SPIB mouse mAb against A549 (1), PC-3 (2), and NIH3T3 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of HeLa cells using SPIB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of paraffin-embedded esophageal cancerwith DAB staining.

Flow Cytometry: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of NIH3T3 cells using SPIB mouse mAb (green) and negative control (red).

Western Blot: Spi-B Antibody (4G5) [NBP2-37458] - Analysis using SPIB mAb against human SPIB recombinant protein. (Expected MW is 32 kDa)

Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of paraffin-embedded ovarian cancer tissues using SPIB mouse mAb with DAB staining.

ELISA: Spi-B Antibody (4G5) [NBP2-37458] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 4G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Spi-B Antibody (4G5) Summary

• Description: Novus Biologicals Mouse Spi-B Antibody (4G5) (NBP2-37458) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-Spi-B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Spi-B (AA: 200-252) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPIB
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 28.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Spi-B Antibody (4G5)

• Spi-B transcription factor (Spi-1/PU.1 related)
• SpiB
• Spi-B
• transcription factor Spi-B

Background

SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1; MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Spi-B Antibody (NBP2-37458)(1)

Publication using NBP2-37458

• Ariga Y, Low S, Hoshino H et al. Expression and Clinical Significance of Spi-B in B-cell Acute Lymphoblastic Leukemia The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2022-09-28 [PMID: 36169277]

Bioinformatics

• Gene Symbol: SPIB