Spi-B Antibody (4G5)

Images

 
Western Blot: Spi-B Antibody (4G5) [NBP2-37458] - Western blot analysis using SPIB mouse mAb against A549 (1), PC-3 (2), and NIH3T3 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Spi-B Antibody (4G5) [NBP2-37458] - Immunofluorescence analysis of HeLa cells using SPIB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry: Spi-B Antibody (4G5) [NBP2-37458] - Immunohistochemical analysis of paraffin-embedded esophageal cancerwith DAB staining.
Flow Cytometry: Spi-B Antibody (4G5) [NBP2-37458] - Flow cytometric analysis of NIH3T3 cells using SPIB mouse mAb (green) and negative control (red).
Western Blot: Spi-B Antibody (4G5) [NBP2-37458] - Western blot analysis using SPIB mAb against human SPIB recombinant protein. (Expected MW is 32 kDa)
Immunohistochemistry: Spi-B Antibody (4G5) [NBP2-37458] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SPIB mouse mAb with DAB staining.
ELISA: Spi-B Antibody (4G5) [NBP2-37458] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
4G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Spi-B Antibody (4G5) Summary

Immunogen
Purified recombinant fragment of human SPIB (AA: 200-252) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPIB
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
Theoretical MW
28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Spi-B Antibody (4G5)

  • Spi-B transcription factor (Spi-1/PU.1 related)
  • SpiB
  • Spi-B
  • transcription factor Spi-B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SPIB
Entrez