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SPIB Products By Gene Name

7 Results for "SPIB"

AF7204

Spi-B Antibody

(8 Publications)

100 μg

AF7204

Spi-B was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Spi-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7204) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Mouse splenocytes were stained with Sheep Anti-Mouse Spi-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7204) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P). Quadrant markers were set based on control antibody staining (Catalog # 5‑001‑A). To facilitate intracellular staining, cells were fixed with paraformadehyde and permeabilized with saponin.

OPGhigh M cells cluster more in cecal patches than in Peyer’s patches.a Whole-mount immunostaining of the FAE of Peyer’s patches (left) and cecal patches (right) for OPG (green) and Spi-B (red). Nuclei were stained with DAPI (blue); scale bars: 50 µm. b Scatter plots of the fluorescence intensities of OPG versus Spi-B. Red dots represent cells stained with anti-Spi-B antibody that was conjugated with HyLyte Fluor 555 and anti-OPG antibody that was conjugated with HyLyte Fluor 647. Blue dots represent background fluorescence intensity of randomly selected non-stained cells. Fluorescence intensities were measured for at least 3000 cells from five FAEs of three mice. c Frequencies of OPGhigh M cells in Peyer’s patches and cecal patches were quantified. *p < 0.05, ***p < 0.005. Student’s t-test, n = 5 FAE from three animals. The source data underlying panels b and c are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31932605), licensed under a CC-BY license. Not internally tested by R&D Systems.

$RANKL–RANK signaling is stimulated in the gut epithelia of Opg−/− mice.a GP2+ cells (red) are more effectively induced in the cecal epithelium (CE) and ileal villi (VE) of Opg−/− mice by RANKL administration. Whole-mount immunohistochemical images of Spi-B (green) and GP2 (red) in the VE and CE of WT (upper panels) and Opg−/− mice (lower panels) treated with either GST (control; left) or GST-RANKL (right). Scale bars: 100 µm. Representative images from three independent experiment are shown. b Quantitative PCR analysis of M-cell marker expression in conventional epithelia from the VE and CE of mice injected with GST (control) or GST-RANKL. Results were normalized to Gapdh expression and are presented relative to the expression in the ileal epithelium from GST-treated mice. Data shown are mean values from three independent experiments (error bars indicate standard deviation). ***p < 0.005, **p < 0.01, *p < 0.05; p values were calculated with the Student’s t-test (n = 3 biologically independent experiments). c, d Nuclear translocation activities of RelB and p52 following RANKL stimulation are enhanced in Opg−/− mice. c Western blot analysis of p100/p52 and RelB in the VE and CE of mice injected with GST or GST-RANKL. Rpt4, a subunit of the 26S proteasome, was used as an internal control for the cytoplasmic fraction. Lamin A/C (Lamin) was used as an internal control for the nuclear fraction. Data are representative of two independent experiments. d Right, single confocal planes of the cecal FAE from WT and Opg−/− mice. FAE monolayers were stained with anti-RelB (green) and anti-Spi-B (red) antibodies and with Hoechst 33342. Left, a bar graph summarizing the proportions of RelB-positive cells among total numbers of M cells (Spi-B-positive cells). ***p < 0.005; p values were calculated with the Student’s t-test (n = 4 biologically independent experiments). Scale bars: 20 µm. The source data underlying panels b and d and non-cropped scan images of western blotting (c) are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31932605), licensed under a CC-BY license. Not internally tested by R&D Systems.$

$RANKL–RANK signaling is stimulated in the gut epithelia of Opg−/− mice.a GP2+ cells (red) are more effectively induced in the cecal epithelium (CE) and ileal villi (VE) of Opg−/− mice by RANKL administration. Whole-mount immunohistochemical images of Spi-B (green) and GP2 (red) in the VE and CE of WT (upper panels) and Opg−/− mice (lower panels) treated with either GST (control; left) or GST-RANKL (right). Scale bars: 100 µm. Representative images from three independent experiment are shown. b Quantitative PCR analysis of M-cell marker expression in conventional epithelia from the VE and CE of mice injected with GST (control) or GST-RANKL. Results were normalized to Gapdh expression and are presented relative to the expression in the ileal epithelium from GST-treated mice. Data shown are mean values from three independent experiments (error bars indicate standard deviation). ***p < 0.005, **p < 0.01, *p < 0.05; p values were calculated with the Student’s t-test (n = 3 biologically independent experiments). c, d Nuclear translocation activities of RelB and p52 following RANKL stimulation are enhanced in Opg−/− mice. c Western blot analysis of p100/p52 and RelB in the VE and CE of mice injected with GST or GST-RANKL. Rpt4, a subunit of the 26S proteasome, was used as an internal control for the cytoplasmic fraction. Lamin A/C (Lamin) was used as an internal control for the nuclear fraction. Data are representative of two independent experiments. d Right, single confocal planes of the cecal FAE from WT and Opg−/− mice. FAE monolayers were stained with anti-RelB (green) and anti-Spi-B (red) antibodies and with Hoechst 33342. Left, a bar graph summarizing the proportions of RelB-positive cells among total numbers of M cells (Spi-B-positive cells). ***p < 0.005; p values were calculated with the Student’s t-test (n = 4 biologically independent experiments). Scale bars: 20 µm. The source data underlying panels b and d and non-cropped scan images of western blotting (c) are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31932605), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF7204U-100UG
Alexa Fluor 405	AF7204V-100UG
Alexa Fluor 488	AF7204G-100UG
Alexa Fluor 532	AF7204X-100UG
Alexa Fluor 594	AF7204T-100UG
Alexa Fluor 647	AF7204R-100UG
Alexa Fluor 700	AF7204N-100UG
Alexa Fluor 750	AF7204S-100UG
Allophycocyanin	AF7204A
Biotin	AF7204B
CoraFluor 1	AF7204CL1
DyLight 350	AF7204D
DyLight 405	AF7204E
DyLight 488	AF7204K
DyLight 550	AF7204L
DyLight 594	AF7204M
DyLight 650	AF7204W
DyLight 680	AF7204Y
DyLight 755	AF7204Z
FITC	AF7204F
HRP	AF7204H
Janelia Fluor 525	AF7204JF525
Janelia Fluor 549	AF7204I
Janelia Fluor 646	AF7204J
Janelia Fluor 669	AF7204JF669
PE	AF7204P
PerCP	AF7204C
mFluor Violet 450 SE	AF7204MFV450
mFluor Violet 500 SE	AF7204MFV500
mFluor Violet 610 SE	AF7204MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7204-SP

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NBP2-37458

Spi-B Antibody (4G5)

(1 Publication)

0.1 ml

NBP2-37458

Western Blot: Spi-B Antibody (4G5) [NBP2-37458] - Analysis using SPIB mouse mAb against A549 (1), PC-3 (2), and NIH3T3 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of HeLa cells using SPIB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of paraffin-embedded esophageal cancerwith DAB staining.

Flow Cytometry: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of NIH3T3 cells using SPIB mouse mAb (green) and negative control (red).

Western Blot: Spi-B Antibody (4G5) [NBP2-37458] - Analysis using SPIB mAb against human SPIB recombinant protein. (Expected MW is 32 kDa)

Immunohistochemistry-Paraffin: Spi-B Antibody (4G5) [NBP2-37458] - Analysis of paraffin-embedded ovarian cancer tissues using SPIB mouse mAb with DAB staining.

ELISA: Spi-B Antibody (4G5) [NBP2-37458] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-70345

Spi-B Antibody

0.1 mg

NBP1-70345

Western Blot: SPIB Antibody [NBP1-70345] - Analysis of Spi-B in human Daudi lysate in the 1) absence and 2) presence of immunizing peptide and 3) on mouse RAW lysate using Spi-B polyclonal antibody at 4 ug/ml.

Reactivity:

Hu, Mu, Bv, Ca, Eq, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Bv, Ca, Eq, Pm, Pm

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6715A

Alt Catalog# IMG-6715A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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MAB7576

Spi-B Antibody (769653)

100 μg

MAB7576

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Spi-B Monoclonal Antibody (Catalog # MAB7576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Spi-B at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7576U-100UG
Alexa Fluor 405	FAB7576V-100UG
Alexa Fluor 488	FAB7576G-100UG
Alexa Fluor 532	FAB7576X-100UG
Alexa Fluor 594	FAB7576T-100UG
Alexa Fluor 647	FAB7576R-100UG
Alexa Fluor 700	FAB7576N-100UG
Alexa Fluor 750	FAB7576S-100UG
Allophycocyanin	FAB7576A
Biotin	FAB7576B
CoraFluor 1	FAB7576CL1
DyLight 350	FAB7576D
DyLight 405	FAB7576E
DyLight 488	FAB7576K
DyLight 550	FAB7576L
DyLight 594	FAB7576M
DyLight 650	FAB7576W
DyLight 680	FAB7576Y
DyLight 755	FAB7576Z
FITC	FAB7576F
HRP	FAB7576H
Janelia Fluor 525	FAB7576JF525
Janelia Fluor 549	FAB7576I
Janelia Fluor 646	FAB7576J
Janelia Fluor 669	FAB7576JF669
PE	FAB7576P
PerCP	FAB7576C
mFluor Violet 450 SE	FAB7576MFV450
mFluor Violet 500 SE	FAB7576MFV500
mFluor Violet 610 SE	FAB7576MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7576-SP

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H00006689-P01

Recombinant Human Spi-B GST (N-Term) Protein

10 μg

H00006689-P01

SDS-Page: Recombinant Human Spi-B Protein [H00006689-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

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NBL1-16399

Spi-B Overexpression Lysate

0.1 mg

NBL1-16399

Western Blot: SPIB Overexpression Lysate (Adult Normal) [NBL1-16399] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPIB.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-98958

Spi-B Antibody - Azide and BSA Free

100 ul

NBP2-98958

Immunoprecipitation: Spi-B Antibody [NBP2-98958] - Spi-B was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate 4 ul anti-Spi-B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Spi-B rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 44 kDa

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor Plus 488	NBP2-98958AFP488
Alexa Fluor Plus 555	NBP2-98958AFP555
Alexa Fluor Plus 594	NBP2-98958AFP594
Alexa Fluor Plus 647	NBP2-98958AFP647
Alexa Fluor Plus 680	NBP2-98958AFP680

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-98958-50ul

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