Spectrin beta 3 Antibody

Western Blot: Spectrin beta 3 Antibody [NB110-58346] - Detection of Human Spectrin beta 3 on HeLa whole cell lysates using NB110-58346. Spectrin beta 3 was also immunoprecipitated by rabbit anti-Spectrin beta 3 antibody NB110-58783.

Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody [NB110-58346] - Detection of Spectrin beta 3 in human prostate epithelium.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Spectrin beta 3 Antibody Summary

• Immunogen: The epitope recognized by this antibody maps to a C-terminal region between residue 2340 and 2390 of Spectrin beta 3 using the numbering given in entry NP_008877.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPTBN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 10-20 ug/ml
  • Immunohistochemistry-Paraffin 10-20 ug/ml
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: This Spectrin beta 3 antibody is useful Immunohistochemistry paraffin embedded sections, Immunoprecipitation and Western blot analysis. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32052742).
• Theoretical MW: 271 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Human. Use in Mouse reported in scientific literature (PMID:32052742).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Spectrin beta 3 Antibody

• beta-III Spectrin
• glutamate transporter EAAT4-associated protein 41
• GTRAP41
• KIAA0302
• SCA5
• SCAR14
• Spectrin beta 3
• spectrin beta chain, brain 2
• Spectrin beta III
• spectrin, beta, non-erythrocytic 2
• Spectrin, non-erythroid beta chain 2
• spinocerebellar ataxia 5
• SPTBN2

Background

Spectrin beta 3 (SPTBN2) belongs to spectrins family of proteins which are essential to mechanical support/structural membrane integrity maintenance in erythrocytes, stabilizing cell-cell contacts, and localizing ion channels as well as cell adhesion molecules within specific subdomains of plasma membranes. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Spectrin beta 3 interacts with EAAT4, the glutamate transporter predominately expressed in Purkinje cells, and stabilizes it at the plasma membrane leading to glutamate clearance from the synaptic cleft, and resulting in both modulation of glutamatergic neurotransmission as well as prevention of glutamate-mediated neurotoxicity. Defective spectrin beta 3 has been established as causing factor in spinocerebellar ataxia type 5 (SCA5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Spectrin beta 3 Antibody (NB110-58346)(1)

Publication using NB110-58346

• Liu CH, Stevens SR, Teliska LH et al. Nodal beta spectrins are required to maintain Na+ channel clustering and axon integrity Elife Feb 13 2020 [PMID: 32052742] (ICC/IF, Mouse)

FAQs for Spectrin beta 3 Antibody (NB110-58346). (Showing 1 - 1 of 1 FAQ).

1. Do you have stability tests done on NB110-58346 if left at RT for several hours ? Customer is doing automatic staining protocol where the antibody needs to stay several hours at RT and they do not get the nice results as with a manual staining, so we are wondering if the antibody would inactivate or get aggregated?
   • Unfortunately, we do not have any stability data on Spectrin beta 3 antibody with catalogue number NB110-58346, however, typical staining protocols involve a couple of hours incubation at room temperature or are performed overnight at 4C. Therefore, I am assuming that the customer should be OK by incubating tissues sections with NB110-58346 for little over a couple of hours without any problems. It may be noted however that the extended incubation with primary antibodies may lead to non-specific binding or overstaining.

Bioinformatics

• Gene Symbol: SPTBN2
• Entrez:
  • 6712(Human)
• Uniprot:
  • O15020(Human)