Western Blot: Spectrin beta 3 Antibody [NB110-58346] - Detection of Human Spectrin beta 3 on HeLa whole cell lysates using NB110-58346. Spectrin beta 3 was also immunoprecipitated by rabbit anti-Spectrin beta 3 antibody ...read more
Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody [NB110-58346] - Detection of Spectrin beta 3 in human prostate epithelium.
This Spectrin beta 3 antibody is useful Immunohistochemistry paraffin embedded sections, Immunoprecipitation and Western blot analysis. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32052742).
271 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human. Use in Mouse reported in scientific literature (PMID:32052742).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Spectrin beta 3 Antibody
glutamate transporter EAAT4-associated protein 41
Spectrin beta 3
spectrin beta chain, brain 2
Spectrin beta III
spectrin, beta, non-erythrocytic 2
Spectrin, non-erythroid beta chain 2
spinocerebellar ataxia 5
Spectrin beta 3 (SPTBN2) belongs to spectrins family of proteins which are essential to mechanical support/structural membrane integrity maintenance in erythrocytes, stabilizing cell-cell contacts, and localizing ion channels as well as cell adhesion molecules within specific subdomains of plasma membranes. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Spectrin beta 3 interacts with EAAT4, the glutamate transporter predominately expressed in Purkinje cells, and stabilizes it at the plasma membrane leading to glutamate clearance from the synaptic cleft, and resulting in both modulation of glutamatergic neurotransmission as well as prevention of glutamate-mediated neurotoxicity. Defective spectrin beta 3 has been established as causing factor in spinocerebellar ataxia type 5 (SCA5).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Spectrin beta 3 Antibody (NB110-58346). (Showing 1 - 1 of 1 FAQ).
Do you have stability tests done on NB110-58346 if left at RT for several hours ? Customer is doing automatic staining protocol where the antibody needs to stay several hours at RT and they do not get the nice results as with a manual staining, so we are wondering if the antibody would inactivate or get aggregated?
Unfortunately, we do not have any stability data on Spectrin beta 3 antibody with catalogue number NB110-58346, however, typical staining protocols involve a couple of hours incubation at room temperature or are performed overnight at 4C. Therefore, I am assuming that the customer should be OK by incubating tissues sections with NB110-58346 for little over a couple of hours without any problems. It may be noted however that the extended incubation with primary antibodies may lead to non-specific binding or overstaining.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.