PKC gamma Antibody

Images

 
Western Blot: PKC gamma Antibody [NBP1-32535] - A. 50 ug mouse brain extract 7.5 % SDS-PAGE PKC gamma antibody dilution: 1:1000
Immunohistochemistry-Paraffin: PKC gamma Antibody [NBP1-32535] - Paraffin-embedded mouse brain. PKC gamma antibody diluted at 1:500.
Western Blot: PKC gamma Antibody [NBP1-32535] - Sample(30 ug whole cell lysate)A:293T7. 5% SDS PAGE, antibody diluted at 1:2000.
Western Blot: PKC gamma Antibody [NBP1-32535] - A. 50 ug rat brain extract 7.5 % SDS-PAGE PKC gamma antibody dilution: 1:1000
Immunohistochemistry-Paraffin: PKC gamma Antibody [NBP1-32535] - Paraffin-embedded Hun7 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PKC gamma Antibody [NBP1-32535] - Paraffin-embedded rat brain. PKC gamma antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RbSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PKC gamma Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PKC gamma. The exact sequence is proprietary.
Predicted Species
Bovine (98%), Rabbit (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKCG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKC gamma Antibody

  • EC 2.7.11
  • EC 2.7.11.13
  • MGC57564
  • PKC gamma
  • PKCC
  • PKCCSCA14
  • PKCG
  • PKC-gamma
  • PKCGprotein kinase C gamma type
  • PRKCG
  • protein kinase C, gamma
  • SCA14

Background

Protein Kinase C gamma (PKC gamma) is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and in regulating intercellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for its autophosphorylation on threonine 655 in the turn loop, and threonine 674 in the hydrophobic loop of the carboxy terminus, a critical step in generating a catalytically mature enzyme. The phosphorylation of the hydrophobic loop in the carboxyl terminus of PKCs is believed to be a key determinant in regulating PKC interaction with PDK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKCG
Entrez