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Ataxin-10 Antibody

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Western Blot: Ataxin-10 Antibody [NBP2-14336] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Western Blot: Ataxin-10 Antibody [NBP2-14336] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: Ataxin-10 Antibody [NBP2-14336] - Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: Ataxin-10 Antibody [NBP2-14336] - Staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Ataxin-10 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KHPESEWPFLIITDLFLKSPELVQAMFPKLNNQERVTLLDLMIAKITSDE PLTKDDIPVFLRHAELIASTF
Specificity
Specificity of human Ataxin-10 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATXN10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Ataxin-10 Protein (NBP2-14336PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Rat (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Ataxin-10 Antibody

  • ataxin 10
  • ataxin-10
  • Brain protein E46 homolog
  • E46L
  • FLJ37990
  • HUMEEP
  • SCA10spinocerebellar ataxia 10
  • Spinocerebellar ataxia type 10 protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-32083
Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250.Blue: Hoechst 33342 staining.
Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10 % SDS-PAGE, and blotted with Ataxin 3 antibody diluted by 1:1000
Species: Hu
Applications: WB, ICC/IF
Ataxin 1 Antibody
(2F5)
NBP1-51689
Immunocytochemistry/Immunofluorescence: Ataxin 1 Antibody (2F5) [NBP1-51689] - Immunofluorescence analysis of NTERA-2 cells using ATXN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Ataxin 1 Antibody (2F5) [NBP1-51689] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues (left) and lung cancer tissues (right) using ATXN1 mouse mAb with DAB staining.
Flow Cytometry: Ataxin 1 Antibody (2F5) [NBP1-51689] - Flow cytometric analysis of Jurket cells using ATXN1 mouse mAb (green) and negative control (purple).
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
PEO1 Antibody
NBP1-90814
Immunohistochemistry: PEO1 Antibody [NBP1-90814] - Staining of human pancreas shows cytoplasmic positivity in islet cells.
Species: Hu
Applications: IHC, IHC-P
PPP2R2B Antibody
NBP2-46667
Western Blot: PPP2R2B Antibody [NBP2-46667] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: PPP2R2B Antibody [NBP2-46667] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PPP2R2B Antibody [NBP2-46667] - Staining of human cerebral cortex shows distinct cytoplasmic and nuclear positivity in neuronal cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Ataxin 7 Antibody
NBP1-42657
Western Blot: Ataxin 7 Antibody [NBP1-42657] - Whole cell lysate (5, 15 and 50 ug) from 293T cells. Antibody used at 0.4 ug/ml.
(1 Publication)
Species: Hu
Applications: IP (-), WB
Ataxin-2 Antibody
NBP1-90063
Western Blot: Ataxin-2 Antibody [NBP1-90063] - Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody [NBP1-90063] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90063] - Staining of human colon, kidney, skeletal muscle and testis using Anti-ATXN2 antibody NBP1-90063 (A) shows similar protein distribution across tissues to independent antibody NBP1-90062 (B).
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
CACNA1A Antibody
NBP2-38049
Immunocytochemistry/Immunofluorescence: CACNA1A Antibody [NBP2-38049] - Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm & the Golgi apparatus.
Immunohistochemistry-Paraffin: CACNA1A Antibody [NBP2-38049] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: CACNA1A Antibody [NBP2-38049] - Staining of human cerebral cortex shows high expression.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Ly-6E Antibody
NBP1-52176
Immunocytochemistry/Immunofluorescence: Ly-6E Antibody [NBP1-52176] - Analysis of paraformaldehyde fixed L929 cells (Mouse fibroblast cell line), permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: Ly-6E Antibody [NBP1-52176] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/Immunofluorescence: Ly-6E Antibody [NBP1-52176] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
(1 Publication)
Species: Mu
Applications: Flow, ICC/IF, PEP-ELISA
TATA binding
protein TBP Antibody
NBP2-12933
Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Staining of Pig Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - NBP2-12933 (1ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Analysis of cell line NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Species: Hu, Mu, Rt, Po
Applications: WB, PEP-ELISA
ATN1 Antibody
NBP1-90044
Immunocytochemistry/Immunofluorescence: ATN1 Antibody [NBP1-90044] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ATN1 Antibody [NBP1-90044] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: ATN1 Antibody [NBP1-90044] - Analysis in human cerebral cortex and liver tissues. Corresponding ATN1 RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
PKC gamma Antibody
NBP1-32535
Western Blot: PKC gamma Antibody [NBP1-32535] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Frozen: PKC gamma Antibody [NBP1-32535] - Frozen-sectioned adult mouse cerebellum. Green: PKC gamma protein stained by PKC gamma antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: PKC gamma Antibody [NBP1-32535] - Sample(30 ug whole cell lysate)A:293T7. 5% SDS PAGE, antibody diluted at 1:2000.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P
c-Myc Antibody
(9E10)
NB600-302
Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.
Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, clone 9E10) at 1:50 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - Analysis of Alexa Fluor (R) 647 conjugate of NB600-302. An intracellular stain was performed on HeLa cells with c-MYC antibody (9E10) NB600-302AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to AF647
(3 Reviews)
(25 Publications)
Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
CCT3 Antibody
NBP1-86562
Western Blot: CCT3 Antibody [NBP1-86562] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CCT3 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunocytochemistry/Immunofluorescence: CCT3 Antibody [NBP1-86562] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-86562] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
FGF14 Antibody
NBP2-55745
Immunocytochemistry/Immunofluorescence: FGF14 Antibody [NBP2-55745] - Staining of human cell line SH-SY5Y shows localization to nucleoli fibrillar center.
Species: Hu
Applications: ICC/IF
PLK1 Antibody
(13E8)
NB100-74502
Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.
Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in WiDr colon carcinoma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: PLK1 Antibody (13E8) [NB100-74502] - Both normal and cancer biopsies of deparaffinized human Colon tissue.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Spectrin beta 3
Antibody (1287A)
NBP2-59668
Western Blot: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - Total protein from Human and Mouse Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Spectrin Beta III in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Spectrin beta 3 (1287A) at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human brain using Spectrin beta III antibody at a 1:1,000 dilution. The primary antibody bound to Spectrin beta III was detected with HRP-DAB detection method and the nuclei were counterstained with hematoxylin. This Spectrin beta III antibody generated a specific cytoplasmic staining in all the cells with strongest signal in neuronal cells.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, Flow-IC
O-GlcNAc
Transferase p110 subu ...
NBP1-32791
Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse tissue extracts (50 ug) was separated by 7.5 % SDS-PAGE, and blotted with O-GlcNAc transferase antibody diluted by 1:2000
Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O- GlcNAc transferase antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Paraffin-embedded rat hind brain. O-GlcNAc transferase antibody diluted at 1:500.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Kv3.3 Antibody
(1C1)
H00003748-M01
Western Blot: Kv3.3 Antibody (1C1) [H00003748-M01] - KCNC3 monoclonal antibody (M01), clone 1C1 Analysis of KCNC3 expression in NIH/3T3.
Immunocytochemistry/Immunofluorescence: Kv3.3 Antibody (1C1) [H00003748-M01] - Analysis of monoclonal antibody to KCNC3 on NIH/3T3 cell. Antibody concentration 10 ug/ml.
(1 Publication)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
IP3R1 Antibody
NBP1-83105
Western Blot: IP3R1 Antibody [NBP1-83105] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-87 MG
Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83105] - Staining of human skin shows no positivity in epidermal cells as expected.
Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83105] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Species: Hu
Applications: WB, IHC, IHC-P
Ataxin-10 Antibody
NBP2-14336
Ataxin-10 Antibody
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol ATXN10