SPARC Antibody

Western Blot: SPARC Antibody [NBP2-27358] - Analysis of human A375 lysate probed with SPARC antibody. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: SPARC Antibody [NBP2-27358] - FFPE human placenta tissue stained with SPARC antibody, peroxidase-conjugate and DAB chromogen.

• Reactivity: Hu, Po, Bv, Pm-Or
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SPARC Antibody Summary

• Description: Novus Biologicals Rabbit SPARC Antibody (NBP2-27358) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 25-85 of human SPARC was used as the immunogen for the antibody. (aa46-63).
• Predicted Species: Porcine (92%), Orangutan (97%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPARC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Western Blot 2-5ug/ml~

Reactivity Notes

The amino acid sequence used as immunogen is 86% homologous in rabbit.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for SPARC Antibody

• Basement-membrane protein 40
• BM-40
• ONcysteine-rich protein
• Osteonectin
• Secreted protein acidic and rich in cysteine
• secreted protein, acidic, cysteine-rich (osteonectin)
• SPARC

Background

SPARC, also called Osteonectin, is an extracellular matrix (ECM) associated glycoprotein. SPARC activates matrix metalloproteinase-2, a key effector of ECM proteolysis, a process linked to enhanced tumor invasion and metastatic potential (1). MMP expression by tumor cells themselves may be representative of more invasive migratory cancers or latter stages of tumor progression (2, 3). SPARC appears to enhance MMP-2 activation at least in invasive breast cancer cell lines. Levels of SPARC expression appear to be directly correlated with progressive cancers not only of breast, but colon, kidney, ovary and brain (4, 5). SPARC specific antibody may establish levels of expression of SPARC providing its' use as a biomarker of late stage or aggressive tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPARC
• Uniprot:
  • P09486()
  • P09486()