SOX9 Antibody [NL557]

SOX9 was detected in immersion fixed U‑251 MG human glioblastoma cell line using Goat Anti-Human SOX9 NorthernLights™ NL557-conjugated Antigen Affinity-purified Polyclonal Antibody (red, Catalog # NL3075R) at 5 µg/mL for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: NL557

SOX9 Antibody [NL557] Summary

• Immunogen: E. coli-derived recombinant human SOX9
  Met1-Lys151
  Accession # P48436
• Specificity: Detects human SOX9 in direct ELISAs and Western blots. In these formats, approximately 10% cross-reactivity with recombinant human (rh) SOX10 is observed and less than 5% cross-reactivity with rhSOX2, rhSOX3, rhSOX7, and rhSOX17 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SOX9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Protect from light.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX9 Antibody [NL557]

• campomelic dysplasia, autosomal sex-reversal
• CMD 1
• CMD1
• CMPD1
• SOX9
• SRA1SRY (sex-determining region Y)-box 9 protein
• SRY (sex determining region Y)-box 9
• SRY-related HMG-box, gene 9
• transcription factor SOX-9

Background

SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX9 Antibody (NL3075R)(1)

Publication using NL3075R

• Veronique Duboc, Fatima A. Sulaiman, Eleanor Feneck, Anna Kucharska, Donald Bell, Muriel Holder-Espinasse et al. Tbx4 function during hindlimb development reveals a novel mechanism to explain the origins of proximal limb defects Development 2021-09-24 [PMID: 34423345]

Bioinformatics

• Gene Symbol: SOX9
• Uniprot:
  • P48436()