SOX17 Antibody (OTI2B6) - Azide and BSA Free

Western Blot: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17.

Immunocytochemistry/Immunofluorescence: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17.

Immunohistochemistry: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299] - Human endometrium tissue using anti-SOX17 mouse monoclonal antibody. Dilution Factor: 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Western Blot: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SOX17 monoclonal antibody.

Immunohistochemistry: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299] - Human endometrium tissue within the normal limits using anti-SOX17 mouse monoclonal antibody. Dilution Factor: 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ICC/IF, IHC
• Clone: OTI2B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SOX17 Antibody (OTI2B6) - Azide and BSA Free Summary

• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 177-414 of human SOX17
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOX17
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Theoretical MW: 43.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SOX17 Antibody (OTI2B6) - Azide and BSA Free

• FLJ22252
• SOX17
• SRY (sex determining region Y)-box 17
• SRY-related HMG-box transcription factor SOX17
• transcription factor SOX-17
• VUR3

Background

The SOX17 gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulationof embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptionalregulator after formi

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOX17