SOX17 Antibody (OTI2B6)

Western Blot: SOX17 Antibody (OTI2B6) [NBP1-47996] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SOX17 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: SOX17 Antibody (OTI2B6) [NBP1-47996] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17.

Immunohistochemistry-Paraffin: SOX17 Antibody (OTI2B6) [NBP1-47996] - Human endometrium tissue using anti-SOX17 mouse monoclonal antibody. Dilution Factor: 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Western Blot: SOX17 Antibody (OTI2B6) [NBP1-47996] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17.

Immunohistochemistry-Paraffin: SOX17 Antibody (OTI2B6) [NBP1-47996] - Human endometrium tissue within the normal limits using anti-SOX17 mouse monoclonal antibody. Dilution Factor: 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ICC/IF, IHC
• Clone: OTI2B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.15 mg/ml

SOX17 Antibody (OTI2B6) Summary

• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 177-414 of human SOX17
• Specificity: This antibody is specific for Homo sapiens SRY (sex determining region Y)-box 17 (SOX17).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOX17
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Theoretical MW: 43.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.15 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SOX17 Antibody (OTI2B6)

• FLJ22252
• SOX17
• SRY (sex determining region Y)-box 17
• SRY-related HMG-box transcription factor SOX17
• transcription factor SOX-17
• VUR3

Background

SOX17 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It may act as a transcriptional regulator after forming a protein complex with other proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX17 Antibody (NBP1-47996)(1)

Publication using NBP1-47996

• Jiang, C;Huang, W;Zhou, Y;Wang, J;Lei, H;Niu, Y;Zhou, R;Zhang, Y; Generating a human induced pluripotent stem cell line (XACHi018-A) from a Timothy syndrome infant carrying heterozygous CACNA1C c.1216G>A (p.G406R) mutation Stem cell research 2024-07-28 [PMID: 39079396]

Bioinformatics

• Gene Symbol: SOX17
• Entrez:
  • 64321(Human)
• Uniprot:
  • Q9H6I2()