SOX10 Antibody (CL4455) - Azide and BSA Free

Analysis in human cerebral cortex and pancreas tissues using NBP3-44116 antibody. Corresponding SOX10 RNA-seq data are presented for the same tissues.

Staining of human breast shows moderate to strong nuclear positivity in glandular cells.

Staining of human cerebral cortex shows strong nuclear positivity in glial cells (oligodendrocytes).

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Staining of human squamous epithelium shows strong nuclear positivity in stratum basale cells.

Staining of mouse embryo E11 shows strong positivity in the developing trigeminal ganglion.

Staining of SK-MEL-30 cells using the Anti-SOX10 monoclonal antibody) .

• Reactivity: Hu, Mu, Fe
• Applications: ICC/IF, IHC
• Clone: CL4455
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SOX10 Antibody (CL4455) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SOX10 Antibody (CL4455) - Azide and BSA Free (NBP2-59050) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P56693, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOX10
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Frozen Validated from a verified customer review.
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Feline reactivity reported from a verified customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for SOX10 Antibody (CL4455) - Azide and BSA Free

• DOM
• MGC15649
• PCWH
• SOX10
• SRY (sex determining region Y)-box 10
• SRY-related HMG-box gene 10
• transcription factor SOX-10
• WS4
• WS4C
• WS4mouse, human homolog of

Background

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOX10