SOS2 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

SOS2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human SOS2
  Met1-Ser384
  Accession # Q07890
• Specificity: Detects human SOS2 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOS2 Antibody [Alexa Fluor® 488]

• FLJ25596
• guanine nucleotide exchange factor
• guanine nucleotide releasing factor
• son of sevenless (Drosophilia) homolog 2
• son of sevenless homolog 2 (Drosophila)
• son of sevenless homolog 2
• SOS2
• SOS-2

Background

SOS2 (Son of sevenless homolog 2) is a 160‑170 kDa protein that regulates Ras signaling. It is widely expressed, and serves as a mediator of guanine nucleotide phosphate exchange. In the resting cell, cytoplasmic SOS2 forms a heterodimer with Grb2, and a heterotrimer with Eps8 and E3b1. Upon RTK activation, the SOS heterodimer is recruited to the cell membrane, where it contacts GDP‑bound Ras. This allows it to facilitate a GTP‑for‑GDP exchange that activates Ras. The heterotrimer interacts with Rac on actin filaments. SOS2 activity parallels that of SOS1. Relative to SOS1, however, SOS2 binds Grb2 with higher affinity and shows less biological activity due to a shorter half‑life. Human SOS2 is 1332 amino acids (aa) in length and contains one histone fold (aa 97‑169), PH (aa 439‑546) and REM (aa 595‑739) domains that interact with Ras, and a Pro‑rich region that binds to Grb2 (aa 1126‑1242).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.