HAF109). A specific band was detected for SOS2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and 293T human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human SOS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOS2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6260-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33487-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55873-25ul | |||
![Immunoprecipitation: SOS2 Antibody [NBP1-05958] - Detection of human SOS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SOS2 antibody NBP1-05958 (lot NBP1-05958-2) used for IP at 6 ug per reaction. SOS2 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-05958-1). For blotting immunoprecipitated SOS2, NBP1-05958 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/SOS2-Antibody-Immunoprecipitation-NBP1-05958-img0004.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: SOS2 Antibody [NBP1-05958] - Detection of human SOS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SOS2 antibody NBP1-05958 (lot NBP1-05958-2) used for IP at 6 ug per reaction. SOS2 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-05958-1). For blotting immunoprecipitated SOS2, NBP1-05958 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![<b>Independent Antibodies Validation. </b>Western Blot: SOS2 Antibody [NBP1-05958] - Whole cell lysate (25 µg) from Jurkat, RKO, CAL-27,PANC-1, and MDA-MB-231 cells prepared using NETN lysisbuffer. Antibody: Affinity purified rabbit anti-SOS2antibody used for WB at 0.4 µg/ml.Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/antibody/nbp1-05958_rabbit-polyclonal-sos2-antibody-western-blot-342025101740..png)
![Western Blot: SOS2 Antibody [NBP1-05958] - Wholecell lysate (25 µg) from CH27, TCMK-1, and BW5147.3cells prepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-SOS2 antibody usedfor WB at 0.4 µg/ml. Detection: Chemiluminescence withan exposure time of 30 seconds.](http://images.novusbio.com/fullsize/antibody/nbp1-05958_rabbit-polyclonal-sos2-antibody-western-blot-342025101847..png)
![Immunohistochemistry-Paraffin: SOS2 Antibody [NBP2-33487] - Staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/SOS2-Antibody-Immunohistochemistry-Paraffin-NBP2-33487-img0003.jpg "Immunohistochemistry-Paraffin: SOS2 Antibody [NBP2-33487] - Staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.")
![Immunocytochemistry/Immunofluorescence: SOS2 Antibody [NBP2-55873] - Staining of human cell line A-431 shows localization to nucleoplasm & vesicles.](http://images.novusbio.com/fullsize/SOS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55873-img0001.jpg "Immunocytochemistry/Immunofluorescence: SOS2 Antibody [NBP2-55873] - Staining of human cell line A-431 shows localization to nucleoplasm & vesicles.")
