Sortilin Antibody (CL6528)

Images

 
Genetic Strategies: Western Blot: Sortilin Antibody (CL6528) [NBP2-76501] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SORT1 antibody. Remaining ...read more
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6528) [NBP2-76501] - Staining of mouse hippocampus shows strong cytoplasmic positivity in both pyramidal neurons and granular cells.
Western Blot: Sortilin Antibody (CL6528) [NBP2-76501] - Analysis in human cell line SK-MEL-30.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Sortilin Antibody (CL6528) [NBP2-76501] - Analysis in human cerebral cortex and tonsil tissues. Corresponding SORT1 RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6528) [NBP2-76501] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6528) [NBP2-76501] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6528) [NBP2-76501] - Staining of human tonsil shows no positivity in lymphoid cells as expected.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6528) [NBP2-76501] - Staining of mouse cerebral cortex shows strong cytoplasmic positivity in neurons.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, KD
Clone
CL6528
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Sortilin Antibody (CL6528) Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LERNCEEKDYTIWLAHSTDPEDYEDGCILGYKEQFLRLRKSSVCQNGRDYVVTKQPSICLCSLEDFLCDFGYYRPENDSKCVEQPELKGHDLEFCLYGREEHLTTNGYRKIPGDKCQGGVNPVREVKD
Epitope
CVEQPELKGH
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SORT1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Sortilin Antibody (CL6528)

  • 100 kDa NT receptor
  • Glycoprotein 95
  • Gp95
  • Gp95LDLCQ6
  • Neurotensin receptor 3
  • NT3NTR3
  • Ntr3
  • SORT1
  • sortilin 1
  • Sortilin

Background

Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SORT1